Single-tube reverse transcription and heminested polymerase chain reaction of hepatitis C virus RNA to detect viremia in serologically negative hemodialysis patients

M. Sampietro, S. Salvadori, N. Corbetta, S. Badalamenti, G. Graziani, G. Fiorelli

Research output: Contribution to journalArticle

Abstract

Detection of hepatitis C virus genome is the only method currently available for detecting viremia in infected individuals. We describe a substantial simplification of current methods for viral RNA reverse transcription and polymerase chain reaction amplification, which allows cDNA synthesis and nested amplification in a single tube; this is achieved through compartmentalization of the reagents for the two reactions with a wax barrier. This procedure retains a high sensitivity: analysis of serum samples from hemodialysis patients, a high-risk group for hepatitis C virus infection, confirmed the presence of the virus in 88% of antibody-positive patients and detected viremia in patients without serological or biochemical evidence of hepatitis C virus infection.

Original languageEnglish
Pages (from-to)52-54
Number of pages3
JournalInternational Journal of Clinical & Laboratory Research
Volume25
Issue number1
DOIs
Publication statusPublished - Mar 1995

Keywords

  • Hemodialysis
  • Hepatitis C virus
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Clinical Biochemistry

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