Sister chromatid exchanges and chromosomal aberrations in mouse cells infected with the Abelson and Moloney leukemia viruses

F. Majone, A. Montaldi, D. Saggioro, L. C. Bianchi, A. G. Levis

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Abstract

'Spontaneous' and mitomycin C (MMC)-induced siter chromatid exchanges (SCE) and chromatid breaks were scored in ANN-1 fibroblasts, a non-producer mouse cell line transformed by the Abelson murine leukemia virus (A-MuLV), a replication defective retrovirus whose genome contains the v-abl oncogene. Normal, non-transformed NIH3T3 fibroblasts were used as control. SCE and chromatid break frequencies in untreated or MMC-treated ANN-1 and NIH3T3 cells were compared with those observed in the same cells after infection with the helper murine Moloney leukemia virus (M-MuLV), which rescues the ability of A-MuLV to replicate in ANN-1 cells. The frequency of spontaneous and MMC-induced SCE were not significantly different in both ANN-1 and NIH3T3 cells, independently of M-MuLV infection. After M-MuLV infection, however, increased 'spontaneous' frequency of SCE and altered susceptibility to the induction of SCE by MMC was observed in both cell lines compared to M-MuLV-uninfected cells. In the case of chromatid breaks, the baseline frequency was not significantly different between the two cell lines both in the presence or in the absence of M-MuLV infection, nor was it significantly increased by M-MuLV, with respect to the value observed in uninfected cells. These results indicate that, at variance with what occurs with SCE, viral replication is not needed to increase the frequency of chromosomal aberrations and that the portion of A-MuLV genome alone is sufficient to increase chromatid breaks but not SCE in ANN-1 cells. Thus, in mouse cells carrying retroviruses, SCE and chromosomal aberrations seem to be independently generated, and influenced by different viral genes.

Original languageEnglish
Pages (from-to)1229-1231
Number of pages3
JournalCarcinogenesis
Volume9
Issue number7
Publication statusPublished - 1988

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Abelson murine leukemia virus
Moloney murine leukemia virus
Sister Chromatid Exchange
Chromatids
Chromosome Aberrations
Mitomycin
Retroviridae
Infection
Fibroblasts
abl Genes
Genome
Helper Viruses
Cell Line
Transformed Cell Line
Viral Genes
Virus Replication

ASJC Scopus subject areas

  • Cancer Research

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Sister chromatid exchanges and chromosomal aberrations in mouse cells infected with the Abelson and Moloney leukemia viruses. / Majone, F.; Montaldi, A.; Saggioro, D.; Bianchi, L. C.; Levis, A. G.

In: Carcinogenesis, Vol. 9, No. 7, 1988, p. 1229-1231.

Research output: Contribution to journalArticle

Majone, F. ; Montaldi, A. ; Saggioro, D. ; Bianchi, L. C. ; Levis, A. G. / Sister chromatid exchanges and chromosomal aberrations in mouse cells infected with the Abelson and Moloney leukemia viruses. In: Carcinogenesis. 1988 ; Vol. 9, No. 7. pp. 1229-1231.
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