Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1

Silvia Schumann, Mineko Terao, Enrico Garattini, Miguel Saggu, Friedhelm Lendzian, Peter Hildebrandt, Silke Leimkühler

Research output: Contribution to journalArticle

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Abstract

Mouse aldehyde oxidase (mAOX1) forms a homodimer and belongs to the xanthine oxidase family of molybdoenzymes which are characterized by an essential equatorial sulfur ligand coordinated to the molybdenum atom. In general, mammalian AOs are characterized by broad substrate specificity and an yet obscure physiological function. To define the physiological substrates and the enzymatic characteristics of mAOX1, we established a system for the heterologous expression of the enzyme in Eschericia coli. The recombinant protein showed spectral features and a range of substrate specificity similar to the native protein purified from mouse liver. The EPR data of recombinant mAOX1 were similar to those of AO from rabbit liver, but differed from the homologous xanthine oxidoreductase enzymes. Site-directed mutagenesis of amino acids Val806, Met884 and Glu1265 at the active site resulted in a drastic decrease in the oxidation of aldehydes with no increase in the oxidation of purine substrates. The double mutant V806E/M884R and the single mutant E1265Q were catalytically inactive enzymes regardless of the aldehyde or purine substrates tested. Our results show that only Glu1265 is essential for the catalytic activity by initiating the base-catalyzed mechanism of substrate oxidation. In addition, it is concluded that the substrate specificity of molybdo-flavoenzymes is more complex and not only defined by the three characterized amino acids in the active site.

Original languageEnglish
Article numbere5348
JournalPLoS One
Volume4
Issue number4
DOIs
Publication statusPublished - Apr 29 2009

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Aldehyde Oxidase
Mutagenesis
site-directed mutagenesis
substrate specificity
Substrate Specificity
Site-Directed Mutagenesis
active sites
aldehydes
Catalytic Domain
oxidation
purines
Amino Acids
Aldehydes
amino acids
mice
Substrates
Enzymes
enzymes
Xanthine Dehydrogenase
xanthine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1. / Schumann, Silvia; Terao, Mineko; Garattini, Enrico; Saggu, Miguel; Lendzian, Friedhelm; Hildebrandt, Peter; Leimkühler, Silke.

In: PLoS One, Vol. 4, No. 4, e5348, 29.04.2009.

Research output: Contribution to journalArticle

Schumann, Silvia ; Terao, Mineko ; Garattini, Enrico ; Saggu, Miguel ; Lendzian, Friedhelm ; Hildebrandt, Peter ; Leimkühler, Silke. / Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX1. In: PLoS One. 2009 ; Vol. 4, No. 4.
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