Six novel variants in the PKLR gene associated with pyruvate kinase deficiency in Argentinian patients

Berenice Milanesio, Carolina Pepe, Lucas A. Defelipe, Silvia Eandi Eberle, Vanesa Avalos Gomez, Alejandro Chaves, Agustina Albero, Fernando Aguirre, Diego Fernandez, Luciana Aizpurua, María Paula Dieuzeide, Adrián Turjanski, Paola Bianchi, Elisa Fermo, Aurora Feliu-Torres

Research output: Contribution to journalArticlepeer-review


Background: Pyruvate kinase deficiency (PKD) is a rare recessive congenital hemolytic anemia caused by mutations in the PKLR gene. The disease shows a marked variability in clinical expression. We studied the molecular features of nine unrelated Argentinian patients with congenital hemolytic anemia associated with erythrocyte pyruvate kinase deficiency. Design and Methods: Routine hematologic investigations were performed to rule out other causes of chronic hemolytic anemia. Sanger sequencing and in-sílico analysis were carried out to identify and characterize the genetics variants. Results: Six different novel missense variants were detected among the 18 studied alleles: c.661 G > C (Asp221His), c.956 G > T (Gly319Val), c.1595 G > C (Arg532Pro), c.347 G > A (Arg116Gln), c.1232 G > T (Gly411Val), c.1021G > A (Gly341Ser). Structural implications of amino-acid substitutions were correlated with the clinical phenotypes seen in the probands. Conclusions: This is the first comprehensive report on molecular characterization of pyruvate kinase deficiency in Argentina and the second from South America that would contribute to our knowledge on the distribution and frequency of PKLR variants in our population but also offer new insights into the interpretation of the effect of PKLR variants and phenotype.

Original languageEnglish
Pages (from-to)26-30
Number of pages5
JournalClinical Biochemistry
Publication statusPublished - May 2021


  • Hemolytic anemia
  • Molecular modelling
  • Novel mutations
  • Pyruvate kinase

ASJC Scopus subject areas

  • Clinical Biochemistry


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