Six-year-old archival chromosome preparations are still good biological reagents for repeated primed in situ labelling (rPRINS)

N. Marziliano, S. Crovella, M. Zuccotti, S. Garagna

Research output: Contribution to journalArticle

Abstract

Six-year-old chromosome preparations of Eulemur coronatus were used for in situ localization of highly repetitive DNA sequences. The application of the repeated primed in situ labelling (rPRINS) technique allowed the detection of positive signals whereas conventional fluorescence in situ hybridization gave negative results on the same archival preparations. This paper reveals the possibility of rescuing archival chromosome preparations for both clinical and comparative cytogenetics. Moreover, the chromosomal localization of the ECOSAT 503 bp highly repetitive DNA sequences were found to localize in the pericentromeric region of every chromosome, with the exception of chromosome 9.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalEuropean journal of histochemistry : EJH
Volume42
Issue number2
Publication statusPublished - 1998

Keywords

  • Archival chromosome preparations
  • Eulemur coronatus
  • rPRINS
  • Satellite DNA

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Animal Science and Zoology
  • Developmental Biology

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