Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration

Luciana De Angelis, Libera Berghella, Marcello Coletta, Laura Lattanzi, Malvina Zanchi, M. Gabriella Cusella-De Angelis, Carola Ponzetto, Giulio Cossu

Research output: Contribution to journalArticle

Abstract

Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met(-/-) embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.

Original languageEnglish
Pages (from-to)869-877
Number of pages9
JournalJournal of Cell Biology
Volume147
Issue number4
DOIs
Publication statusPublished - Nov 15 1999

Fingerprint

Aorta
Regeneration
Muscles
Growth
Blood Vessels
Skeletal Muscle
Embryonic Structures
Somites
Muscle Development
Mesoderm
Muscle Cells
Vertebrates
Extremities
Clone Cells
Transplantation

Keywords

  • Endothelial cells
  • Multipotent progenitors
  • Myogenesis
  • Satellite cells
  • Vascular-endothelial cadherin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration. / De Angelis, Luciana; Berghella, Libera; Coletta, Marcello; Lattanzi, Laura; Zanchi, Malvina; Cusella-De Angelis, M. Gabriella; Ponzetto, Carola; Cossu, Giulio.

In: Journal of Cell Biology, Vol. 147, No. 4, 15.11.1999, p. 869-877.

Research output: Contribution to journalArticle

De Angelis, L, Berghella, L, Coletta, M, Lattanzi, L, Zanchi, M, Cusella-De Angelis, MG, Ponzetto, C & Cossu, G 1999, 'Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration', Journal of Cell Biology, vol. 147, no. 4, pp. 869-877. https://doi.org/10.1083/jcb.147.4.869
De Angelis, Luciana ; Berghella, Libera ; Coletta, Marcello ; Lattanzi, Laura ; Zanchi, Malvina ; Cusella-De Angelis, M. Gabriella ; Ponzetto, Carola ; Cossu, Giulio. / Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration. In: Journal of Cell Biology. 1999 ; Vol. 147, No. 4. pp. 869-877.
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