Abstract
Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met(-/-) embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.
| Original language | English |
|---|---|
| Pages (from-to) | 869-877 |
| Number of pages | 9 |
| Journal | Journal of Cell Biology |
| Volume | 147 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Nov 15 1999 |
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Keywords
- Endothelial cells
- Multipotent progenitors
- Myogenesis
- Satellite cells
- Vascular-endothelial cadherin
ASJC Scopus subject areas
- Cell Biology
Cite this
Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration. / De Angelis, Luciana; Berghella, Libera; Coletta, Marcello; Lattanzi, Laura; Zanchi, Malvina; Cusella-De Angelis, M. Gabriella; Ponzetto, Carola; Cossu, Giulio.
In: Journal of Cell Biology, Vol. 147, No. 4, 15.11.1999, p. 869-877.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration
AU - De Angelis, Luciana
AU - Berghella, Libera
AU - Coletta, Marcello
AU - Lattanzi, Laura
AU - Zanchi, Malvina
AU - Cusella-De Angelis, M. Gabriella
AU - Ponzetto, Carola
AU - Cossu, Giulio
PY - 1999/11/15
Y1 - 1999/11/15
N2 - Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met(-/-) embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.
AB - Skeletal muscle in vertebrates is derived from somites, epithelial structures of the paraxial mesoderm, yet many unrelated reports describe the occasional appearance of myogenic cells from tissues of nonsomite origin, suggesting either transdifferentiation or the persistence of a multipotent progenitor. Here, we show that clonable skeletal myogenic cells are present in the embryonic dorsal aorta of mouse embryos. This finding is based on a detailed clonal analysis of different tissue anlagen at various developmental stages. In vitro, these myogenic cells show the same morphology as satellite cells derived from adult skeletal muscle, and express a number of myogenic and endothelial markers. Surprisingly, the latter are also expressed by adult satellite cells. Furthermore, it is possible to clone myogenic cells from limbs of mutant c-Met(-/-) embryos, which lack appendicular muscles, but have a normal vascular system. Upon transplantation, aorta-derived myogenic cells participate in postnatal muscle growth and regeneration, and fuse with resident satellite cells. The potential of the vascular system to generate skeletal muscle cells may explain observations of nonsomite skeletal myogenesis and raises the possibility that a subset of satellite cells may derive from the vascular system.
KW - Endothelial cells
KW - Multipotent progenitors
KW - Myogenesis
KW - Satellite cells
KW - Vascular-endothelial cadherin
UR - http://www.scopus.com/inward/record.url?scp=0033571046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033571046&partnerID=8YFLogxK
U2 - 10.1083/jcb.147.4.869
DO - 10.1083/jcb.147.4.869
M3 - Article
C2 - 10562287
AN - SCOPUS:0033571046
VL - 147
SP - 869
EP - 877
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 4
ER -