Skewed B cell differentiation affects lymphoid organogenesis but not T cell-mediated autoimmunity

E. Colombo, P. Tentorio, S. Musio, K. Rajewsky, R. Pedotti, S. Casola, C. Farina

Research output: Contribution to journalArticle

Abstract

Summary: B cell receptor (BCR) signalling determines B cell differentiation and may potentially alter T cell-mediated immune responses. In this study we used two transgenic strains of BCR-deficient mice expressing Epstein-Barr virus latent membrane protein (LMP)2A in B cells, where either follicular and marginal zone differentiation (DHLMP2A mice) or B-1 cell development (VHLMP2A mice) were supported, and evaluated the effects of skewed B lymphocyte differentiation on lymphoid organogenesis and T cell responses in vivo. Compared to wild-type animals, both transgenic strains displayed alterations in the composition of lymphoid organs and in the dynamics of distinct immune cell subsets following immunization with the self-antigen PLP185-206. However, ex-vivoT cell proliferation to PLP185-206 peptide measured in immunized DHLMP2A and VHLMP2A mice was similar to that detected in immunized control mice. Further, clinical expression of experimental autoimmune encephalitis in both LMP2A strains was identical to that of wild-type mice. In conclusion, mice with skewed B cell differentiation driven by LMP2A expression in BCR-negative B cells do not show changes in the development of a T cell mediated disease model of autoimmunity, suggesting that compensatory mechanisms support the generation of T cell responses.

Original languageEnglish
Pages (from-to)58-65
Number of pages8
JournalClinical and Experimental Immunology
Volume176
Issue number1
DOIs
Publication statusPublished - Apr 2014

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Keywords

  • B cell receptor
  • B cells
  • EAE
  • LMP2A

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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