Slug/β-catenin-dependent proinflammatory phenotype in hypoxic breast cancer stem cells

Gianluca Storci, Sara Bertoni, Sabrina De Carolis, Alessio Papi, Marina Nati, Claudio Ceccarelli, Chiara Pirazzini, Paolo Garagnani, Alberto Ferrarini, Genny Buson, Massimo Delledonne, Michelangelo Fiorentino, Elisa Capizzi, Elisa Gruppioni, Mario Taffurelli, Donatella Santini, Claudio Franceschi, Giuseppe Bandini, Francesca Bonifazi, Massimiliano Bonafé

Research output: Contribution to journalArticlepeer-review


Cancer stem cell survival relies on the activation of inflammatory pathways, which is speculatively triggered by cell autonomous mechanisms or by microenvironmental stimuli. Here, we observed that hypoxic bone marrow stroma-derived transforming growth factor-β 1 promotes the growth of human breast cancer stem cells as mammospheres. The ensuing Slug-dependent serine 139 phosphorylation of the DNA damage sensor H2AX in breast cancer stem cells induces tumor necrosis factor-α and IL-8 mRNAs, whose stability is enhanced by cytoplasmic β-catenin. β-Catenin also up-regulates and binds miR-221, reducing the stability of the miR-221 targets Rad51 and ERα mRNAs. Our data show that the Slug/β-catenin-dependent activation of DNA damage signaling triggered by the hypoxic microenvironment sustains the proinflammatory phenotype of breast cancer stem cells.

Original languageEnglish
Pages (from-to)1688-1697
Number of pages10
JournalAmerican Journal of Pathology
Issue number5
Publication statusPublished - Nov 2013

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


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