Slug gene expression supports human osteoblast maturation

Elisabetta Lambertini, Gina Lisignoli, Elena Torreggiani, Cristina Manferdini, Elena Gabusi, Tiziana Franceschetti, Letizia Penolazzi, Roberto Gambari, Andrea Facchini, Roberta Piva

Research output: Contribution to journalArticle

Abstract

This study aims to define the function of Slug transcription factor in human normal osteoblasts (hOBs). To date, Slug is considered exclusively a marker of malignancy in bone tissue. Here, we identified, for the first time, a role for Slug in hOBs using a knockdown approach. We demonstrated that Slug is positively correlated with osteoblast markers, including Runx2, osteopontin, osteocalcin, Collagen type 1, Wnt/β-catenin signaling mediators, and mineral deposition. At the same time, Slug silencing potentiates the expression of Sox-9, a factor indispensable for chondrogenic development. These data, with the finding that Slug is in vivo recruited by the promoters of Runx2 and Sox-9 genes, suggest that, in hOBs, Slug may act both as positive and negative transcriptional regulator of Runx2 and Sox-9 genes, respectively. In summary, our results support the hypothesis that Slug functions as a novel regulator of osteoblast activity and may be considered a new factor required for osteoblast maturation.

Original languageEnglish
Pages (from-to)3641-3653
Number of pages13
JournalCellular and Molecular Life Sciences
Volume66
Issue number22
DOIs
Publication statusPublished - Nov 2009

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Keywords

  • Human osteoblasts
  • Runx2
  • Slug
  • Sox-9
  • Wnt signaling

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

Lambertini, E., Lisignoli, G., Torreggiani, E., Manferdini, C., Gabusi, E., Franceschetti, T., Penolazzi, L., Gambari, R., Facchini, A., & Piva, R. (2009). Slug gene expression supports human osteoblast maturation. Cellular and Molecular Life Sciences, 66(22), 3641-3653. https://doi.org/10.1007/s00018-009-0149-5