Small-animal radionuclide luminescence imaging of thyroid and salivary glands with Tc99m-pertechnetate

Federico Boschi, Marco Pagliazzi, Barbara Rossi, Maria Paola Cecchini, Giancarlo Gorgoni, Matteo Salgarello, Antonello E. Spinelli

Research output: Contribution to journalArticle

Abstract

The in vitro and in vivo detection of visible photons from radioisotopes using optical techniques is a fastgrowing field in molecular imaging. 99mTc-pertechnetate is used as an alternative to 123I in imaging of the thyroid and is generally imaged with gamma cameras or single photon emission tomography instruments. The uptake in the thyroid tissue is mediated by the sodium-iodide symporter (NIS), a glycoprotein that actively mediates iodide transport into the thyroid follicular cells and several extrathyroidal tissues. The luminescence of the gamma emitter 99mTc-pertechnetate in order to visualize its biodistribution in healthy small living animals by using a commercial optical imaging system is investigated. Here we show that in Nu/Nu mice, the uptake of 99mTc-pertechnetate in the thyroid gland and in salivary glands is very detectable by using radionuclide luminescence imaging. We also found light emission from the stomach in accordance with the literature. The localization of the light signals in the anatomical regions where the radiopharmaceutical is expected, confirmed by resections, shows that it is possible to image NIS-expressing tissues.

Original languageEnglish
Article number076005
JournalJournal of Biomedical Optics
Volume18
Issue number7
DOIs
Publication statusPublished - Jul 2013

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Keywords

  • 99mTc-pertechnetate
  • radioisotope luminescence imaging
  • small animals
  • thyroid imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Medicine(all)

Cite this

Boschi, F., Pagliazzi, M., Rossi, B., Cecchini, M. P., Gorgoni, G., Salgarello, M., & Spinelli, A. E. (2013). Small-animal radionuclide luminescence imaging of thyroid and salivary glands with Tc99m-pertechnetate. Journal of Biomedical Optics, 18(7), [076005]. https://doi.org/10.1117/1.JBO.18.7.076005