Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae

Alexander A. Mironov, Galina V. Beznoussenko, Paolo Nicoziani, Oliviano Martella, Alvar Trucco, Hee Seok Kweon, Daniele Di Giandomenico, Roman S. Polishchuk, Aurora Fusella, Pietro Lupetti, Eric G. Berger, Willie J C Geerts, Abraham J. Koster, Koert N J Burger, Alberto Luini

Research output: Contribution to journalArticlepeer-review

Abstract

Procollagen (PC)-I aggregates transit through the Golgi complex without leaving the lumen of Golgi cisternae. Based on this evidence, we have proposed that PC-I is transported across the Golgi stacks by the cisternal maturation process. However, most secretory cargoes are small, freely diffusing proteins, thus raising the issue whether they move by a transport mechanism different than that used by PC-I. To address this question we have developed procedures to compare the transport of a small protein, the G protein of the vesicular stomatitis virus (VSVG), with that of the much larger PC-I aggregates in the same cell. Transport was followed using a combination of video and EM, providing high resolution in time and space. Our results reveal that PC-I aggregates and VSVG move synchronously through the Golgi at indistinguishable rapid rates. Additionally, not only PC-I aggregates (as confirmed by ultrarapid cryofixation), but also VSVG, can traverse the stack without leaving the cisternal lumen and without entering Golgi vesicles in functionally relevant amounts. Our findings indicate that a common mechanism independent of anterograde dissociative carriers is responsible for the traffic of small and large secretory cargo across the Golgi stack.

Original languageEnglish
Pages (from-to)1225-1238
Number of pages14
JournalJournal of Cell Biology
Volume155
Issue number7
DOIs
Publication statusPublished - Dec 24 2001

Keywords

  • Golgi complex
  • Intracellular traffic
  • Procollagen
  • Transport vesicles
  • VSVG

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae'. Together they form a unique fingerprint.

Cite this