Small noncoding RNAs in cells transformed by human T-cell leukemia virus type 1: A lole for a tRNA fragment as a primer for reverse transcriptase

Katia Ruggero, Alessandro Guffanti, Alberto Corradin, Varun Kumar Sharma, Gianluca De Bellis, Giorgio Corti, Angela Grassi, Paola Zanovello, Vincenzo Bronte, Vincenzo Ciminale, Donna M. D'Agostino

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Abstract

The present study employed mass sequencing of small RNA libraries to identify the repertoire of small noncoding RNAs expressed in normal CD+4 T cells compared to cells transformed with human T-cell leukemia virus type 1 (HTLV-1), the causative agent of adult T-cell leukemia/lymphoma (ATLL). The results revealed distinct patterns of microRNA expression in HTLV-1-infected CD+4 T-cell lines with respect to their normal counterparts. In addition, a search for virus-encoded microRNAs yielded 2 sequences that originated from the plus strand of the HTLV-1 genome. Several sequences derived from tRNAs were expressed at substantial levels in both uninfected and infected cells. One of the most abundant tRNA fragments (tRF-3019) was derived from the 3' end of tRNA-proline. tRF-3019 exhibited perfect sequence complementarity to the primer binding site of HTLV-1. The results of an in vitro reverse transcriptase assay verified that tRF-3019 was capable of priming HTLV-1 reverse transcriptase. Both tRNA-proline and tRF-3019 were detected in virus particles isolated from HTLV-1-infected cells. These findings suggest that tRF-3019 may play an important role in priming HTLV-1 reverse transcription and could thus represent a novel target to control HTLV-1 infection.

Original languageEnglish
Pages (from-to)3612-3622
Number of pages11
JournalJournal of Virology
Volume88
Issue number7
DOIs
Publication statusPublished - 2014

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ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Ruggero, K., Guffanti, A., Corradin, A., Sharma, V. K., De Bellis, G., Corti, G., Grassi, A., Zanovello, P., Bronte, V., Ciminale, V., & D'Agostino, D. M. (2014). Small noncoding RNAs in cells transformed by human T-cell leukemia virus type 1: A lole for a tRNA fragment as a primer for reverse transcriptase. Journal of Virology, 88(7), 3612-3622. https://doi.org/10.1128/JVI.03483-13