Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles

Michela Rigoni, Giampietro Schiavo, Anne E. Weston, Paola Caccin, Federica Allegrini, Maria Pennuto, Flavia Valtorta, Cesare Montecucco, Ornella Rossetto

Research output: Contribution to journalArticle

Abstract

The mechanisms of action of four snake presynaptic phospholipase A2 neurotoxins were investigated in cultured neurons isolated from various parts of the rat brain. Strikingly, physiological concentrations of notexin, β-bungarotoxin, taipoxin or textilotoxin induced a dose-dependent formation of discrete bulges at various sites of neuronal projections. Neuronal bulging was paralleled by the redistribution of the two synaptic vesicle markers synaptophysin I (SypI) and vesicle-attached membrane protein 2 (VAMP2) to the bulges, and by the exposure of the luminal domain of synaptotagmin on the cell surface. These neurotoxins induced glutamate release from cultured neurons similarly to the known evoked release of acetylcholine from neuromuscular junctions. In addition, partial fragmentation of F-actin and neurofilaments was observed in neurons, but not in astrocytes. These findings indicate that these snake presynaptic neurotoxins act with by same mechanism and that the observed phenotype results from the fusion of synaptic vesicles with the plasma membrane not balanced by an adequate membrane retrieval. These changes closely resemble those occurring at neuromuscular junctions of intoxicated animals and fully qualify these primary neuronal cultures as pertinent models for studying the molecular mode of action of these neurotoxins.

Original languageEnglish
Pages (from-to)3561-3570
Number of pages10
JournalJournal of Cell Science
Volume117
Issue number16
DOIs
Publication statusPublished - Jul 15 2004

Fingerprint

Snakes
Synaptic Vesicles
Phospholipases A2
Exocytosis
Neurotoxins
Neurites
Neuromuscular Junction
Neurons
Synaptotagmins
Bungarotoxins
Synaptophysin
Molecular Models
Intermediate Filaments
Astrocytes
Acetylcholine
Actins
Glutamic Acid
Membrane Proteins
Cell Membrane
Phenotype

Keywords

  • Neurotransmitter release
  • Phospholipase A2
  • Snake presynaptic neurotoxins
  • Synaptic vesicle recycling
  • Synaptic vesicles

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rigoni, M., Schiavo, G., Weston, A. E., Caccin, P., Allegrini, F., Pennuto, M., ... Rossetto, O. (2004). Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles. Journal of Cell Science, 117(16), 3561-3570. https://doi.org/10.1242/jcs.01218

Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles. / Rigoni, Michela; Schiavo, Giampietro; Weston, Anne E.; Caccin, Paola; Allegrini, Federica; Pennuto, Maria; Valtorta, Flavia; Montecucco, Cesare; Rossetto, Ornella.

In: Journal of Cell Science, Vol. 117, No. 16, 15.07.2004, p. 3561-3570.

Research output: Contribution to journalArticle

Rigoni, M, Schiavo, G, Weston, AE, Caccin, P, Allegrini, F, Pennuto, M, Valtorta, F, Montecucco, C & Rossetto, O 2004, 'Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles', Journal of Cell Science, vol. 117, no. 16, pp. 3561-3570. https://doi.org/10.1242/jcs.01218
Rigoni, Michela ; Schiavo, Giampietro ; Weston, Anne E. ; Caccin, Paola ; Allegrini, Federica ; Pennuto, Maria ; Valtorta, Flavia ; Montecucco, Cesare ; Rossetto, Ornella. / Snake presynaptic neurotoxins with phospholipase A2 activity induce punctate swellings of neurites and exocytosis of synaptic vesicles. In: Journal of Cell Science. 2004 ; Vol. 117, No. 16. pp. 3561-3570.
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