SNAP-25 regulates spine formation through postsynaptic binding to p140Cap

Romana Tomasoni, Daniele Repetto, Raffaella Morini, Chiara Elia, Fabrizio Gardoni, Monica Di Luca, Emilia Turco, Paola Defilippi, Michela Matteoli

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Synaptosomal-associated protein of 25 kDa (SNAP-25) is a member of the Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) protein family, required for exocytosis of synaptic vesicles and regulation of diverse ion channels. Here, we show that acute reduction of SNAP-25 expression leads to an immature phenotype of dendritic spines that are, consistently, less functional. Conversely, over-expression of SNAP-25 results in an increase in the density of mature, Postsynaptic Density protein 95 (PSD-95)-positive spines. The regulation of spine morphogenesis by SNAP-25 depends on the protein's ability to bind both the plasma membrane and the adaptor protein p140Cap, a key protein regulating actin cytoskeleton and spine formation. We propose that SNAP-25 allows the organization of the molecular apparatus needed for spine formation by recruiting and stabilizing p140Cap.

Original languageEnglish
Pages (from-to)2136
Number of pages1
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013

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Synaptosomal-Associated Protein 25
spine
Spine
proteins
Proteins
SNARE Proteins
Dendritic Spines
Synaptic Vesicles
Exocytosis
Cell membranes
Actin Cytoskeleton
Ion Channels
Morphogenesis
Actins
Blood Proteins
Membrane Proteins
Cell Membrane
Phenotype
phenotype
attachment

ASJC Scopus subject areas

  • Medicine(all)

Cite this

SNAP-25 regulates spine formation through postsynaptic binding to p140Cap. / Tomasoni, Romana; Repetto, Daniele; Morini, Raffaella; Elia, Chiara; Gardoni, Fabrizio; Di Luca, Monica; Turco, Emilia; Defilippi, Paola; Matteoli, Michela.

In: Nature Communications, Vol. 4, 2013, p. 2136.

Research output: Contribution to journalArticle

Tomasoni, Romana ; Repetto, Daniele ; Morini, Raffaella ; Elia, Chiara ; Gardoni, Fabrizio ; Di Luca, Monica ; Turco, Emilia ; Defilippi, Paola ; Matteoli, Michela. / SNAP-25 regulates spine formation through postsynaptic binding to p140Cap. In: Nature Communications. 2013 ; Vol. 4. pp. 2136.
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AU - Di Luca, Monica

AU - Turco, Emilia

AU - Defilippi, Paola

AU - Matteoli, Michela

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