TY - JOUR
T1 - Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast
AU - Pessina, Stefania
AU - Tsiarentsyeva, Viktoryia
AU - Busnelli, Sara
AU - Vanoni, Marco
AU - Alberghina, Lilia
AU - Coccetti, Paola
PY - 2010/6/1
Y1 - 2010/6/1
N2 - The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. the lack of Snf1 α-catalytic subunit downregulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G
1/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G
1/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G
1 to S transition, differing from the reported AMpK role in controlling the G
1/S transition in multicellular eukaryotes.
AB - The Saccharomyces cerevisiae Snf1 protein kinase has been reported to be required for adaptation to glucose limitation and for growth on non-fermentable carbon sources. Here we present novel findings indicating that Snf1, the key regulator of cellular energy, is also involved in yeast cell cycle control. the lack of Snf1 α-catalytic subunit downregulates the growth rate and CLB5 expression, delaying Sld2 phosphorylation and G
1/S transition, in cells grown in 2%, but not in 5% glucose. A non-phosphorylatable Snf1 rescues the slow growth phenotype, whereas a wild type or a phosphomimetic mutant is required to rescue growth rate and the G
1/S delay. Using either Snf1 or Swi6 as a bait, a specific interaction of Snf1 with Swi6, the regulatory subunit of MBF, was detected. In conclusion, this report describes a previously unrecognized role for Snf1 in transcriptional modulation of the G
1 to S transition, differing from the reported AMpK role in controlling the G
1/S transition in multicellular eukaryotes.
KW - AMPK/Snf1
KW - Cell cycle
KW - Clb5
KW - G /S transition
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=77953594906&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953594906&partnerID=8YFLogxK
U2 - 10.4161/cc.9.11.11847
DO - 10.4161/cc.9.11.11847
M3 - Article
C2 - 20505334
AN - SCOPUS:77953594906
VL - 9
SP - 2189
EP - 2200
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 11
ER -