Solar UV radiation: Differential effectiveness of UVB subcomponents in causing cell death, micronucleus induction and delayed expression of heritable damage in human hybrid cells

D. Bettega, P. Calzolari, F. Belloni, F. Di Lena, S. Genchi, M. Lupi, P. Massariello, S. Orsini, L. Tallone, D. Tomasoni, P. Ubezio, J. L. Redpath

Research output: Contribution to journalArticle

Abstract

Purpose: To determine the effectiveness of two UV spectra with different UVB components for cell kill and micronucleus induction in irradiated human HeLa × skin fibroblast (CGL1) hybrid cells and their progeny. To determine the presence of reactive oxygen species (ROS) in the progeny of the irradiated cells at various post-irradiation times and their relationship with induced delayed biological effects. Material and methods: A commercial solar ultraviolet simulator was used. Two different filters were employed: the first transmitted radiation with λ>284nm and the second radiation with λ > 293 nm. The resulting spectra have different UVB components (2 between 284 and 320nm, 19 W/m2, and between 293 and 320nm, 13 W/m2) and the same UVA component (λ between 320 and 400 nm, 135 W/m2). CGL1 cells were irradiated with various doses. Clonogenic survival and micronucleus formation were scored in the irradiated cells and their progeny. ROS were detected by incubation of cultures at various post-irradiation times with dichlorodihydrofluorescein diacetate followed by flow cytometric measurement of the final product, dichlorofluorescein. Results: The biological effectiveness of the λ>284nm spectrum was higher by a factor of 3 compared to the λ > 293 nm spectrum for cell kill, and by a factor of 5 for micronucleus induction. No delayed cell death or micronucleus formation was found in the progeny of cells exposed to λ > 293 nm, while a large and dose-dependent effect was found in the progeny of cells exposed to λ> 284 nm for both of these endpoints. ROS levels above those in unirradiated controls were found only in the progeny of cells exposed to the λ > 284 nm spectrum. Conclusions: The spectrum with λ>284nm was more effective than that with λ > 293 nm for induction of cell kill and micronucleus formation in the directly irradiated cells as well as induction of delayed effects in the progeny in the form of delayed reproductive death and micronucleus formation. The presence of ROS in the progeny of the irradiated cells may be the cause of the delayed effects.

Original languageEnglish
Pages (from-to)963-970
Number of pages8
JournalInternational Journal of Radiation Biology
Volume77
Issue number9
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

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