Solid lipid nanoparticles carrying oligonucleotides inhibit vascular endothelial growth factor expression in rat glioma models

Andrea M. Brioschi, Sara Calderoni, Luca G. Pradotto, Marilena Guido, Alberto Strada, Francesco Zenga, Carlo Alberto Benech, Franco Benech, Loredana Serpe, Gian Paolo Zara, Claudia Musicanti, Alessandro Ducati, Maria Rosa Gasco, Alessandro Mauro

Research output: Contribution to journalArticle

Abstract

Vascular endothelial growth factor (VEGF) was proposed as a target for antiangiogenetic therapy in high-grade gliomas. We tested the efficacy of solid lipid nanoparticles (SLNs) carrying VEGF antisense oligonucleotides to down-regulate VEGF expression in an in vitro and in vivo rat glioma model. In vitro: rat C6 glioma cells under hypoxic conditions were treated with VEGF antisense oligonucleotides, either free or vehiculated by SLNs at different concentrations for 24 and 48 hours. At Western blot analysis, cellular VEGF expression was significantly reduced (p

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalJournal of Nanoneuroscience
Volume1
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • Antisense oligonucleotides
  • C6 glioma cells
  • Glioma
  • Solid lipid nanoparticles
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)

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  • Cite this

    Brioschi, A. M., Calderoni, S., Pradotto, L. G., Guido, M., Strada, A., Zenga, F., Benech, C. A., Benech, F., Serpe, L., Zara, G. P., Musicanti, C., Ducati, A., Gasco, M. R., & Mauro, A. (2009). Solid lipid nanoparticles carrying oligonucleotides inhibit vascular endothelial growth factor expression in rat glioma models. Journal of Nanoneuroscience, 1(1), 65-74. https://doi.org/10.1166/jns.2009.007