TY - JOUR
T1 - Solid-phase extraction of fluoropyrimidine derivatives on a copper-modified strong cation exchanger
T2 - determination of doxifluridine, 5-fluorouracil and its main metabolites in serum by high-performance liquid chromatography with ultraviolet detection
AU - Guerrieri, A.
AU - Palmisano, F.
AU - Zambonin, P. G.
AU - De Lena, M.
AU - Lorusso, V.
PY - 1993/7/23
Y1 - 1993/7/23
N2 - A copper-modified strong cation exchange stationary phase was used for the solid phase extraction of the antineoplastic drug doxifluridine (5′-deoxy-5-fluorouridine) and some of its main metabolites from human serum. Sorption of fluoropyrimidine derivatives due to complex formation with copper(II) was maximal at pH values around neutrality. Analytes were eluted by ligand exchange with ammonia. The average recoveries ranged from 70% to 108%. Reversed-phase chromatography with ultraviolet detection was used for the separation and quantitation of the analytes. The overall procedure has been applied to serum samples from patients receiving doxifluridine chemotherapy, and a pharmacokinetic profile has been derived.
AB - A copper-modified strong cation exchange stationary phase was used for the solid phase extraction of the antineoplastic drug doxifluridine (5′-deoxy-5-fluorouridine) and some of its main metabolites from human serum. Sorption of fluoropyrimidine derivatives due to complex formation with copper(II) was maximal at pH values around neutrality. Analytes were eluted by ligand exchange with ammonia. The average recoveries ranged from 70% to 108%. Reversed-phase chromatography with ultraviolet detection was used for the separation and quantitation of the analytes. The overall procedure has been applied to serum samples from patients receiving doxifluridine chemotherapy, and a pharmacokinetic profile has been derived.
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U2 - 10.1016/0378-4347(93)80423-2
DO - 10.1016/0378-4347(93)80423-2
M3 - Article
C2 - 8376540
AN - SCOPUS:0027237697
VL - 617
SP - 71
EP - 77
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1
ER -