Solid-phase extraction of fluoropyrimidine derivatives on a copper-modified strong cation exchanger: determination of doxifluridine, 5-fluorouracil and its main metabolites in serum by high-performance liquid chromatography with ultraviolet detection

A. Guerrieri, F. Palmisano, P. G. Zambonin, M. De Lena, V. Lorusso

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A copper-modified strong cation exchange stationary phase was used for the solid phase extraction of the antineoplastic drug doxifluridine (5′-deoxy-5-fluorouridine) and some of its main metabolites from human serum. Sorption of fluoropyrimidine derivatives due to complex formation with copper(II) was maximal at pH values around neutrality. Analytes were eluted by ligand exchange with ammonia. The average recoveries ranged from 70% to 108%. Reversed-phase chromatography with ultraviolet detection was used for the separation and quantitation of the analytes. The overall procedure has been applied to serum samples from patients receiving doxifluridine chemotherapy, and a pharmacokinetic profile has been derived.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume617
Issue number1
DOIs
Publication statusPublished - Jul 23 1993

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Solid Phase Extraction
High performance liquid chromatography
Metabolites
Fluorouracil
Cations
Copper
High Pressure Liquid Chromatography
Derivatives
Serum
Pharmacokinetics
Chemotherapy
Reverse-Phase Chromatography
Chromatography
Ammonia
Antineoplastic Agents
Sorption
Ligands
Drug Therapy
Recovery
doxifluridine

ASJC Scopus subject areas

  • Chemistry(all)
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

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abstract = "A copper-modified strong cation exchange stationary phase was used for the solid phase extraction of the antineoplastic drug doxifluridine (5′-deoxy-5-fluorouridine) and some of its main metabolites from human serum. Sorption of fluoropyrimidine derivatives due to complex formation with copper(II) was maximal at pH values around neutrality. Analytes were eluted by ligand exchange with ammonia. The average recoveries ranged from 70{\%} to 108{\%}. Reversed-phase chromatography with ultraviolet detection was used for the separation and quantitation of the analytes. The overall procedure has been applied to serum samples from patients receiving doxifluridine chemotherapy, and a pharmacokinetic profile has been derived.",
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T2 - determination of doxifluridine, 5-fluorouracil and its main metabolites in serum by high-performance liquid chromatography with ultraviolet detection

AU - Guerrieri, A.

AU - Palmisano, F.

AU - Zambonin, P. G.

AU - De Lena, M.

AU - Lorusso, V.

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