Solid state nanopores for gene expression profiling

V. Mussi, P. Fanzio, L. Repetto, G. Firpo, U. Valbusa, P. Scaruffi, S. Stigliani, G. P. Tonini

Research output: Contribution to journalArticle

Abstract

Recently, nanopore technology has been introduced for genome analysis. Here we show results related to the possibility of preparing "engineered solid state nanopores". The nanopores were fabricated on a suspended Si3N4 membrane by Focused Ion Beam (FIB) drilling and chemically functionalized in order to covalently bind oligonucleotides (probes) on their surface. Our data show the stable effect of DNA attachment on the ionic current measured through the nanopore, making it possible to conceive and develop a selective biosensor for gene expression profiling.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalSuperlattices and Microstructures
Volume46
Issue number1-2
DOIs
Publication statusPublished - Jul 2009

Keywords

  • Biosensor
  • Functionalization
  • Gene expression
  • Ionic current
  • Nanopore

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Materials Science(all)

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    Mussi, V., Fanzio, P., Repetto, L., Firpo, G., Valbusa, U., Scaruffi, P., Stigliani, S., & Tonini, G. P. (2009). Solid state nanopores for gene expression profiling. Superlattices and Microstructures, 46(1-2), 59-63. https://doi.org/10.1016/j.spmi.2008.09.003