Solid state nanopores for gene expression profiling

V. Mussi; P. Fanzio; L. Repetto; G. Firpo; U. Valbusa; P. Scaruffi; S. Stigliani; G. P. Tonini

doi:10.1016/j.spmi.2008.09.003

Solid state nanopores for gene expression profiling

V. Mussi, P. Fanzio, L. Repetto, G. Firpo, U. Valbusa, P. Scaruffi, S. Stigliani, G. P. Tonini

A.O.U. San Martino - IST, Istituto Nazionale Ricerca sul Cancro (GENOVA)

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, nanopore technology has been introduced for genome analysis. Here we show results related to the possibility of preparing "engineered solid state nanopores". The nanopores were fabricated on a suspended Si₃N₄ membrane by Focused Ion Beam (FIB) drilling and chemically functionalized in order to covalently bind oligonucleotides (probes) on their surface. Our data show the stable effect of DNA attachment on the ionic current measured through the nanopore, making it possible to conceive and develop a selective biosensor for gene expression profiling.

Original language	English
Pages (from-to)	59-63
Number of pages	5
Journal	Superlattices and Microstructures
Volume	46
Issue number	1-2
DOIs	https://doi.org/10.1016/j.spmi.2008.09.003
Publication status	Published - Jul 2009

Keywords

Biosensor
Functionalization
Gene expression
Ionic current
Nanopore

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics
Materials Science(all)

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10.1016/j.spmi.2008.09.003

Cite this

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AU - Mussi, V.

AU - Fanzio, P.

AU - Repetto, L.

AU - Firpo, G.

AU - Valbusa, U.

AU - Scaruffi, P.

AU - Stigliani, S.

AU - Tonini, G. P.

PY - 2009/7

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AB - Recently, nanopore technology has been introduced for genome analysis. Here we show results related to the possibility of preparing "engineered solid state nanopores". The nanopores were fabricated on a suspended Si3N4 membrane by Focused Ion Beam (FIB) drilling and chemically functionalized in order to covalently bind oligonucleotides (probes) on their surface. Our data show the stable effect of DNA attachment on the ionic current measured through the nanopore, making it possible to conceive and develop a selective biosensor for gene expression profiling.

KW - Biosensor

KW - Functionalization

KW - Gene expression

KW - Ionic current

KW - Nanopore

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Solid state nanopores for gene expression profiling

Abstract

Keywords

ASJC Scopus subject areas

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