Soluble human MHC class I molecules induce soluble Fas ligand secretion and trigger apoptosis in activated CD8+ Fas (CD95)+ T lymphocytes

Francesco Puppo, Paola Contini, Massimo Ghio, Sabrina Brenci, Marco Scudeletti, Gilberto Filaci, Soldano Ferrone, Francesco Indiveri

Research output: Contribution to journalArticle

Abstract

In the present study, we have evaluated the apoptotic effect of soluble human MHC class I (sHLA-I) antigens on CD8+ T lymphocytes. sHLA-I antigens and β2-microglobulin-free HLA class I heavy chains, isolated from serum, induced apoptosis on phytohemagglutinin-activated CD8+ T lymphocytes in autologous and allogeneic combinations. The extent of CD8+ T cell apoptosis depends on the degree of activation, time of incubation with sHLA-I antigens and amount of sHLA-I antigens added to the cultures. Apoptosis is induced by the interaction of Fas (CD95)+ cells with soluble Fas ligand which is released following binding of sHLA-I antigens to CD8 molecules. These results suggest that sHLA-I antigens may regulate immune responses by inducing apoptosis in activated CD8+ T cells.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalInternational Immunology
Volume12
Issue number2
Publication statusPublished - 2000

Keywords

  • HLA
  • Immunomodulation

ASJC Scopus subject areas

  • Immunology

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    Puppo, F., Contini, P., Ghio, M., Brenci, S., Scudeletti, M., Filaci, G., Ferrone, S., & Indiveri, F. (2000). Soluble human MHC class I molecules induce soluble Fas ligand secretion and trigger apoptosis in activated CD8+ Fas (CD95)+ T lymphocytes. International Immunology, 12(2), 195-203.