TY - JOUR
T1 - Soluble intercellular adhesion molecule-1 in ovarian follicles
T2 - Production by granulosa luteal cells and levels in follicular fluid
AU - Viganò, Paola
AU - Fusi, Francesco
AU - Gaffuri, Barbara
AU - Bonzi, Viviana
AU - Ferrari, Augusto
AU - Vignali, Mario
PY - 1998/4
Y1 - 1998/4
N2 - Objective: To determine the concentration of the soluble form of intercellular adhesion molecule (ICAM)-1 in granulosa luteal cell-conditioned media and in follicular fluid (FF). Design: Granulosa cells and FF samples were obtained at the time of oocyte retrieval for IVF. In 10 women, a total of 33 fluids were obtained from individual follicles, whereas in 70 women, the follicular aspirates were pooled. Setting: Clinica 'L. Mangiagalli' and Reproductive Center, San Raffaele Hospital, Milan, Italy. Patient(s): Eighty women referred for IVF for tubal factor or male factor infertility. Intervention(s): Women underwent ovarian hyperstimulation. Main Outcome Measure(s): Soluble ICAM-1 was measured by an ELISA, and its levels were correlated with follicular size, the number of retrieved oocytes, and the number of follicles with a diameter of > 15 mm. Result(s): The concentration of soluble ICAM-1 in granulosa luteal cell-conditioned media was 17.8 ± 1.8 ng/5 x 105 cells. Interleukin-1β can stimulate soluble ICAM-1 release in a dose-dependent manner. A significant pesitive correlation was demonstrated between levels of soluble ICAM-1 in pooled FF and the number of retrieved oocytes or the number of follicles with a diameter of > 15 min. Conclusion(s): Soluble ICAM-I can be released by granulosa luteal cells and can be detected in FF after ovarian hyperstimulation. Levels of soluble ICAM- 1 in FF correlate directly with some indices of ovarian function.
AB - Objective: To determine the concentration of the soluble form of intercellular adhesion molecule (ICAM)-1 in granulosa luteal cell-conditioned media and in follicular fluid (FF). Design: Granulosa cells and FF samples were obtained at the time of oocyte retrieval for IVF. In 10 women, a total of 33 fluids were obtained from individual follicles, whereas in 70 women, the follicular aspirates were pooled. Setting: Clinica 'L. Mangiagalli' and Reproductive Center, San Raffaele Hospital, Milan, Italy. Patient(s): Eighty women referred for IVF for tubal factor or male factor infertility. Intervention(s): Women underwent ovarian hyperstimulation. Main Outcome Measure(s): Soluble ICAM-1 was measured by an ELISA, and its levels were correlated with follicular size, the number of retrieved oocytes, and the number of follicles with a diameter of > 15 mm. Result(s): The concentration of soluble ICAM-1 in granulosa luteal cell-conditioned media was 17.8 ± 1.8 ng/5 x 105 cells. Interleukin-1β can stimulate soluble ICAM-1 release in a dose-dependent manner. A significant pesitive correlation was demonstrated between levels of soluble ICAM-1 in pooled FF and the number of retrieved oocytes or the number of follicles with a diameter of > 15 min. Conclusion(s): Soluble ICAM-I can be released by granulosa luteal cells and can be detected in FF after ovarian hyperstimulation. Levels of soluble ICAM- 1 in FF correlate directly with some indices of ovarian function.
KW - Follicle
KW - Oocyte
KW - Ovarian function
KW - Soluble intercellular adhesion molecule (sICAM)- 1
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U2 - 10.1016/S0015-0282(97)00565-7
DO - 10.1016/S0015-0282(97)00565-7
M3 - Article
C2 - 9548172
AN - SCOPUS:0031969145
VL - 69
SP - 774
EP - 779
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 4
ER -