Soluble proteins from chemosensory organs of Eurycantha calcarata (Insects, Phasmatodea)

Silvana Marchese, Sergio Angeli, Annapaola Andolfo, Andrea Scaloni, Anna Brandazza, Mario Mazza, Jean François Picimbon, Walter S. Leal, Paolo Pelosi

Research output: Contribution to journalArticlepeer-review

Abstract

Three related nucleotide sequences, encoding mature proteins of 108-113 amino acids, have been obtained from antennal cDNA of the Phasmid Eurycantha calcarata. Among these, one is also expressed in the tarsi as demonstrated by N-terminal sequence and mass spectrometric analyses of protein samples isolated from both organs. PCR experiments performed with specific primers, showed that this species is also expressed in the mouth organs and in the cuticle, while the other two are antennal specific. All three isoforms are similar to Drosophila OS-D and other proteins reported in several insect orders, but one of them is significantly different from the other two. The best conserved elements are the N-terminal region and the four cysteine residues. Accurate ESMS measurements indicated that all cysteines are involved in two disulphide bonds and ruled out the occurrence of additional posttranslational modifications. Polyclonal antibodies, raised against the purified protein, did not react with proteins of the same class expressed in another Phasmid species, Carausius morosus, and in the orthopteran Schistocerca gregaria, nor did antibodies against these proteins recognise those of E. calcarata. (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)1091-1098
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume30
Issue number11
DOIs
Publication statusPublished - 2000

Keywords

  • Chemosensory proteins
  • Eurycantha calcarata
  • Odorant-binding proteins
  • Phasmids
  • RACE
  • Sequence analysis

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

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