Somatostatin inhibits the stimulatory effect of secretin on pancreatic cancer cell growth in vitro

Maria Fiera Panozzo, Daniela Basso, Annarosa Del Mistro, Roberta Bertorelle, Annalisa Stefani, Mario Plebani

Research output: Contribution to journalArticle

Abstract

Cell growth is the result of intracellular regulatory processes which may be activated or inhibited by extracellular factors, such as hormones. These phenomena can be modified in neoplastic conditions. This study was undertaken in order to: (1) evaluate the role of secretin and/or somatostatin in influencing either proliferation or intracellular cAMP content of a human pancreatic cancer cell line (MIA PaCa2), and (2) identify the role of pertussis toxin sensitive C proteins in mediating the effects of secretin and/or somatostatin. Secretin, but not somatostatin, was found to stimulate MIA PaCa2 cell proliferation. Somatostatin (100 nmol/l) inhibited secretin stimulated cell growth. When cells were pretreated with pertussis toxin, secretin alone or secretin plus somatostatin had no effect on cell growth, thus indicating that membrane G proteins, blocked by pertussis toxin, are involved in internalizing the secretin signal. In conclusion, secretin stimulates pancreatic cancer cell growth through a pertussis toxin sensitive C protein mechanism. Somatostatin abolishes the stimulatory effect of secretin on cell proliferation.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalPathophysiology
Volume4
Issue number1
DOIs
Publication statusPublished - 1997

Keywords

  • cell culture
  • cell proliferation
  • cyclic AMP
  • G proteins
  • growth factors
  • pertussis toxin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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