Somatostatin release is enhanced in the hippocampus of partially and fully kindled rats

A. Vezzani, A. Monno, M. Rizzi, A. Galli, M. Barrios, R. Samanin

Research output: Contribution to journalArticlepeer-review

Abstract

The release of somatostatin (somatostatin-like immunoreactivity) from hippocampal slices during the development of hippocampal kindling in rats was measured under resting and depolarizing conditions. Preliminary experiments in naive rats showed that the spontaneous efflux of somatostatin (4.0 ± 0.3 fmol/ml every 10 min) was independent of external Ca2+ but was reduced to 71.5 ± 6% of baseline (P <0.05) during 20 min incubation with 5 μM tetrodotoxin. Neuronal depolarization with 25, 50 and 100 mM KC1 induced a Ca2+-dependent somatostatin release, respectively 4.3 ± 0.4, 16.7 ± 1.6 and 22.0 ± 1.3 times baseline (P <0.01). Veratridine caused a dose-dependent Ca2+ and tetrodotoxin (5 μM) sensitive release ranging from 6.5 ± 0.1 to 13.0 ± 1.4 times baseline at 1.4μM and 50 μM respectively (P <0.01). One week after the last of three consecutive stage 5 seizures (full seizure expression) or 48 h after the last stage 2 stimulation (preconvulsive stage), 50 mM KC1-induced somatostatin release was significantly higher (1.8 ± 0.1, P

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalNeuroscience
Volume51
Issue number1
DOIs
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Neuroscience(all)

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