Some parameters for a cell cycle cytotopochemical study.

M. G. Manfredi Romanini, D. Formenti, A. Fraschini, C. Pellicciari, C. Redi, E. Scherini

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Abstract

With the Feulgen-Naphthol Yellow double reaction we dosed, simultaneously on a cell, [1] the DNA nuclear content (as Feulgen positive material), [2] the protein cell content and [3] the Feulgen chromatin area; the degree of condensation is expressed by the ratio [1]/[3]. The evolution of the lymphocytes culture at different times of PHA stimulation was in this way studied with the aim of defining the existence of cell subpopulations pertaining at different phases or subphases of the cycle.

Original languageEnglish
Pages (from-to)581-587
Number of pages7
JournalGegenbaurs morphologisches Jahrbuch
Volume127
Issue number4
Publication statusPublished - 1981

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Manfredi Romanini, M. G., Formenti, D., Fraschini, A., Pellicciari, C., Redi, C., & Scherini, E. (1981). Some parameters for a cell cycle cytotopochemical study. Gegenbaurs morphologisches Jahrbuch, 127(4), 581-587.