Some patients with antiphospholipid syndrome express hitherto undescribed antibodies to cardiolipin-binding proteins

P. Rampazzo, A. Biasiolo, J. Garin, A. Rosato, C. Betterle, A. Ruffatti, V. Pengo

Research output: Contribution to journalArticle

Abstract

Contrary to infective anticardiolipin (aCL) antibodies, autoimmune aCL antibodies react with phospholipids (PL) mainly via binding to the plasma glycoprotein cofactor β2-Glycoprotein I (β2GPI). While there is a well-documented link between the risk of thrombosis and the presence of β2GPI-dependent anticardiolipin antibodies, the pathological impact of other antiphospholipid antibodies is less clear. By means of cardiolipin affinity-chromatography, we isolated and identified 3 CL-binding proteins, complement component C4, complement factor H and a kallikrein-sensitive glycoprotein, and tested for the presence of autoantibodies against these proteins in patients with antiphospholipid syndrome (APS), systemic lupus erythematosus (SLE) and other autoimmune diseases. High titers of autoantibodies to C4 as compared to age- and sex-matched healthy controls were present in 3 of 26 patients with APS, and weak titers were found in 2 of 26 patients with SLE and in none of 26 patients with other autoimmune diseases. Autoantibodies to complement factor H were found in 4 APS, 3 SLE and none of the other autoimmune patients. Autoantibodies to kallikrein-sensitive glycoprotein were detected in 6 APS patients, 1 SLE patient, and 1 patient with another autoimmune disease. A close relationship between these antibodies was found, suggesting their origin from a common macro-molecular complex. However, no relationship with anti-β2GPI antibodies was found, with the three patients with higher levels of autoantibodies having a low titer of anti-β2GPI antibodies. In conclusion, some patients with APS harbor circulating antibodies to other CL-binding proteins which might be useful to further characterize these patients.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalThrombosis and Haemostasis
Volume85
Issue number1
Publication statusPublished - 2001

Keywords

  • β-glycoprotein I
  • Complement component C4
  • Complement factor H
  • Plasma kallikrein-sensitive glycoprotein

ASJC Scopus subject areas

  • Hematology

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    Rampazzo, P., Biasiolo, A., Garin, J., Rosato, A., Betterle, C., Ruffatti, A., & Pengo, V. (2001). Some patients with antiphospholipid syndrome express hitherto undescribed antibodies to cardiolipin-binding proteins. Thrombosis and Haemostasis, 85(1), 57-62.