Some questions about Eurocollins solution used for lung preservation

L. Spaggiari, P. Carbognani, M. Rusca, R. Alfieri, P. G. Petronini, L. Cattelani, P. Salcuni, A. F. Borghetti, P. Bobbio

Research output: Contribution to journalArticle

Abstract

Currently, Eurocollins' (EC) solution (high-potassium concentration) is the most widely clinically used pulmonary perfusate. However, recently, experimental studies have reported an increase of the lung ischemic period using low-potassium solutions. The purpose of our study, is to investigate the influence of the EC ionic composition and the effect of hyperosmolarity due to the glucose concentration on isolated alveolar type II epithelial cells. Pneumocytes type II were isolated from pathogen free Wistar rats using the modified Dobbs' method. Cells were incubated for 6 hours at 4°C in EC, Collins (CL) and Ringer Lactate (RL) solutions. After that, cellular viability was evaluated by analysis of the protein synthesis assay by measuring the 35 S methionine uptake during an incorporation period of one hour at 37°C (picomol 35 S met/mg proteins/h). Mean ± standard deviation and Student 't'-test were used for data presentation and results comparison. Cellular viability at time 0 (control) before cellular incubation was 3.93 ± 0.38. After 6 hours at 4°C the results were respectively as follows: EC = 2.16 ± 0.13; CL = 2.63 ± 0; RL = 3.21 ± 0.04. Our results suggest that the low-potassium extracellular type solution (RL) shows a protection on isolated type II epithelial cells statistically significant (p <0.05) if compared with EC solution. Moreover CL solution, that has the same ionic composition EC but without glucose, presents a less cytotoxic effect on incubated cells than EC, confirming a deleterious influence of solution hyperosmolarity.

Original languageEnglish
Pages (from-to)149-152
Number of pages4
JournalJournal of Cardiovascular Surgery
Volume35
Issue numberSUPPL. 1-6
Publication statusPublished - 1994

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Keywords

  • Extracellular solution
  • Intracellular solution
  • Lung preservation
  • Lung transplantation
  • Pneumocytes type II

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Spaggiari, L., Carbognani, P., Rusca, M., Alfieri, R., Petronini, P. G., Cattelani, L., Salcuni, P., Borghetti, A. F., & Bobbio, P. (1994). Some questions about Eurocollins solution used for lung preservation. Journal of Cardiovascular Surgery, 35(SUPPL. 1-6), 149-152.