TY - JOUR
T1 - Southern Blot Analysis of ALL-1 Rearrangements at Chromosome 11q23 in Acute Leukemia
AU - Coco, Francesco Lo
AU - Mandelli, Franco
AU - Breccia, Massimo
AU - Annino, Luciana
AU - Guglielmi, Cesare
AU - Petti, Maria C.
AU - Testi, Anna M.
AU - Alimena, Giuliana
AU - Croce, Carlo M.
AU - Canaani, Eli
PY - 1993
Y1 - 1993
N2 - The chromosome 11q23 band is a genetic region frequently involved in nonrandom karyotypic abnormalities of acute leukemia. A genomic locus named ALL-1 or MLL, where 11q23 breakpoints are clustered, has been recently cloned and characterized. We have made use of an ALL-1 -specific probe in Southern blot experiments to analyze the configuration of this gene in a large series of acute leukemia patients, representative of all different myeloid and lymphoid subtypes.Nine of 145 cases (62%) showed abnormal ALL-1 restriction fragments in leukemic DNAs. Of these nine cases, five patients in whom karyotypic data were available displayed chromosome llq23 aberrations, including t(4;ll) (three cases) and t(9;ll) (two cases). Immunophenotypic and morphocytochemical characterization of ALL-l-rearranged acute leukemia revealed prevalence of poorly differentiated B lymphoid and/or monoblastic features. Considering the whole series, ALL-1 rearrangements were significantly associated with female sex, higher white blood cell counts at presentation, and very poor clinical outcome. The presence of residual disease was molecularly documented in one case at the time of clinical remission after induction treatment and was followed by early relapse. We conclude that ALL-1 rearrangements are new molecular markers of human leukemia with considerable diagnostic and prognostic relevance.
AB - The chromosome 11q23 band is a genetic region frequently involved in nonrandom karyotypic abnormalities of acute leukemia. A genomic locus named ALL-1 or MLL, where 11q23 breakpoints are clustered, has been recently cloned and characterized. We have made use of an ALL-1 -specific probe in Southern blot experiments to analyze the configuration of this gene in a large series of acute leukemia patients, representative of all different myeloid and lymphoid subtypes.Nine of 145 cases (62%) showed abnormal ALL-1 restriction fragments in leukemic DNAs. Of these nine cases, five patients in whom karyotypic data were available displayed chromosome llq23 aberrations, including t(4;ll) (three cases) and t(9;ll) (two cases). Immunophenotypic and morphocytochemical characterization of ALL-l-rearranged acute leukemia revealed prevalence of poorly differentiated B lymphoid and/or monoblastic features. Considering the whole series, ALL-1 rearrangements were significantly associated with female sex, higher white blood cell counts at presentation, and very poor clinical outcome. The presence of residual disease was molecularly documented in one case at the time of clinical remission after induction treatment and was followed by early relapse. We conclude that ALL-1 rearrangements are new molecular markers of human leukemia with considerable diagnostic and prognostic relevance.
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M3 - Article
C2 - 8339294
AN - SCOPUS:0027205347
VL - 53
SP - 3800
EP - 3803
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 16
ER -