Abstract
Glioblastoma, the most aggressive cerebral tumor, is invariably lethal. Glioblastoma cells express several genes typical of normal neural stem cells. One of them, SOX2, is a master gene involved in sustaining self-renewal of several stem cells, in particular neural stem cells. To investigate its role in the aberrant growth of glioblastoma, we silenced SOX2 in freshly derived glioblastoma tumor-initiating cells (TICs). Our results indicate that SOX2 silenced glioblastoma TICs, despite the many mutations they have accumulated, stop proliferating and lose tumorigenicity in immunodeficient mice. SOX2 is then also fundamental for maintenance of the self-renewal capacity of neural stem cells when they have acquired cancer properties. SOX2, or its immediate downstream effectors, would then be an ideal target for glioblastoma therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 40-48 |
| Number of pages | 9 |
| Journal | Stem Cells |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2009 |
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Keywords
- Glioblastoma
- SOX2 gene silencing
- Tumor-initiating cells
- Tumorigenesis
ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Molecular Medicine
Cite this
SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity. / Gangemi, Rosaria Maria Rita; Griffero, Fabrizio; Marubbi, Daniela; Perera, Marzia; Capra, Maria Cristina; Malatesta, Paolo; Ravetti, Gian Luigi; Zona, Gian Luigi; Daga, Antonio; Corte, Giorgio.
In: Stem Cells, Vol. 27, No. 1, 01.2009, p. 40-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity
AU - Gangemi, Rosaria Maria Rita
AU - Griffero, Fabrizio
AU - Marubbi, Daniela
AU - Perera, Marzia
AU - Capra, Maria Cristina
AU - Malatesta, Paolo
AU - Ravetti, Gian Luigi
AU - Zona, Gian Luigi
AU - Daga, Antonio
AU - Corte, Giorgio
PY - 2009/1
Y1 - 2009/1
N2 - Glioblastoma, the most aggressive cerebral tumor, is invariably lethal. Glioblastoma cells express several genes typical of normal neural stem cells. One of them, SOX2, is a master gene involved in sustaining self-renewal of several stem cells, in particular neural stem cells. To investigate its role in the aberrant growth of glioblastoma, we silenced SOX2 in freshly derived glioblastoma tumor-initiating cells (TICs). Our results indicate that SOX2 silenced glioblastoma TICs, despite the many mutations they have accumulated, stop proliferating and lose tumorigenicity in immunodeficient mice. SOX2 is then also fundamental for maintenance of the self-renewal capacity of neural stem cells when they have acquired cancer properties. SOX2, or its immediate downstream effectors, would then be an ideal target for glioblastoma therapy.
AB - Glioblastoma, the most aggressive cerebral tumor, is invariably lethal. Glioblastoma cells express several genes typical of normal neural stem cells. One of them, SOX2, is a master gene involved in sustaining self-renewal of several stem cells, in particular neural stem cells. To investigate its role in the aberrant growth of glioblastoma, we silenced SOX2 in freshly derived glioblastoma tumor-initiating cells (TICs). Our results indicate that SOX2 silenced glioblastoma TICs, despite the many mutations they have accumulated, stop proliferating and lose tumorigenicity in immunodeficient mice. SOX2 is then also fundamental for maintenance of the self-renewal capacity of neural stem cells when they have acquired cancer properties. SOX2, or its immediate downstream effectors, would then be an ideal target for glioblastoma therapy.
KW - Glioblastoma
KW - SOX2 gene silencing
KW - Tumor-initiating cells
KW - Tumorigenesis
UR - http://www.scopus.com/inward/record.url?scp=59849125142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59849125142&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2008-0493
DO - 10.1634/stemcells.2008-0493
M3 - Article
C2 - 18948646
AN - SCOPUS:59849125142
VL - 27
SP - 40
EP - 48
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 1
ER -