Spastin Binds to Lipid Droplets and Affects Lipid Metabolism

Chrisovalantis Papadopoulos, Genny Orso, Giuseppe Mancuso, Marija Herholz, Sentiljana Gumeni, Nimesha Tadepalle, Christian Jüngst, Anne Tzschichholz, Astrid Schauss, Stefan Höning, Aleksandra Trifunovic, Andrea Daga, Elena I. Rugarli

Research output: Contribution to journalArticle

Abstract

Mutations in SPAST, encoding spastin, are the most common cause of autosomal dominant hereditary spastic paraplegia (HSP). HSP is characterized by weakness and spasticity of the lower limbs, owing to progressive retrograde degeneration of the long corticospinal axons. Spastin is a conserved microtubule (MT)-severing protein, involved in processes requiring rearrangement of the cytoskeleton in concert to membrane remodeling, such as neurite branching, axonal growth, midbody abscission, and endosome tubulation. Two isoforms of spastin are synthesized from alternative initiation codons (M1 and M87). We now show that spastin-M1 can sort from the endoplasmic reticulum (ER) to pre- and mature lipid droplets (LDs). A hydrophobic motif comprised of amino acids 57 through 86 of spastin was sufficient to direct a reporter protein to LDs, while mutation of arginine 65 to glycine abolished LD targeting. Increased levels of spastin-M1 expression reduced the number but increased the size of LDs. Expression of a mutant unable to bind and sever MTs caused clustering of LDs. Consistent with these findings, ubiquitous overexpression of Dspastin in Drosophila led to bigger and less numerous LDs in the fat bodies and increased triacylglycerol levels. In contrast, Dspastin overexpression increased LD number when expressed specifically in skeletal muscles or nerves. Downregulation of Dspastin and expression of a dominant-negative variant decreased LD number in Drosophila nerves, skeletal muscle and fat bodies, and reduced triacylglycerol levels in the larvae. Moreover, we found reduced amount of fat stores in intestinal cells of worms in which the spas-1 homologue was either depleted by RNA interference or deleted. Taken together, our data uncovers an evolutionarily conserved role of spastin as a positive regulator of LD metabolism and open up the possibility that dysfunction of LDs in axons may contribute to the pathogenesis of HSP.

Original languageEnglish
Article numbere1005149
JournalPLoS Genetics
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1 2015

Fingerprint

Lipid Metabolism
lipid metabolism
droplets
droplet
metabolism
lipid
lipids
Hereditary Spastic Paraplegia
fat
Fat Body
Drosophila
Axons
Skeletal Muscle
Triglycerides
mutation
Retrograde Degeneration
muscle
Microtubule Proteins
axons
Lipid Droplets

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Papadopoulos, C., Orso, G., Mancuso, G., Herholz, M., Gumeni, S., Tadepalle, N., ... Rugarli, E. I. (2015). Spastin Binds to Lipid Droplets and Affects Lipid Metabolism. PLoS Genetics, 11(4), [e1005149]. https://doi.org/10.1371/journal.pgen.1005149

Spastin Binds to Lipid Droplets and Affects Lipid Metabolism. / Papadopoulos, Chrisovalantis; Orso, Genny; Mancuso, Giuseppe; Herholz, Marija; Gumeni, Sentiljana; Tadepalle, Nimesha; Jüngst, Christian; Tzschichholz, Anne; Schauss, Astrid; Höning, Stefan; Trifunovic, Aleksandra; Daga, Andrea; Rugarli, Elena I.

In: PLoS Genetics, Vol. 11, No. 4, e1005149, 01.04.2015.

Research output: Contribution to journalArticle

Papadopoulos, C, Orso, G, Mancuso, G, Herholz, M, Gumeni, S, Tadepalle, N, Jüngst, C, Tzschichholz, A, Schauss, A, Höning, S, Trifunovic, A, Daga, A & Rugarli, EI 2015, 'Spastin Binds to Lipid Droplets and Affects Lipid Metabolism', PLoS Genetics, vol. 11, no. 4, e1005149. https://doi.org/10.1371/journal.pgen.1005149
Papadopoulos C, Orso G, Mancuso G, Herholz M, Gumeni S, Tadepalle N et al. Spastin Binds to Lipid Droplets and Affects Lipid Metabolism. PLoS Genetics. 2015 Apr 1;11(4). e1005149. https://doi.org/10.1371/journal.pgen.1005149
Papadopoulos, Chrisovalantis ; Orso, Genny ; Mancuso, Giuseppe ; Herholz, Marija ; Gumeni, Sentiljana ; Tadepalle, Nimesha ; Jüngst, Christian ; Tzschichholz, Anne ; Schauss, Astrid ; Höning, Stefan ; Trifunovic, Aleksandra ; Daga, Andrea ; Rugarli, Elena I. / Spastin Binds to Lipid Droplets and Affects Lipid Metabolism. In: PLoS Genetics. 2015 ; Vol. 11, No. 4.
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