Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons

Andrea Menegon, Claudia Verderio, Chiara Leoni, Fabio Benfenati, Andrew J. Czernik, Paul Greengard, Michela Matteoli, Flavia Valtorta

Research output: Contribution to journalArticle

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Abstract

We have studied Ca2+/calmodulin-dependent protein kinase II (CaMKII) isoform distribution and activity in embryonic hippocampal neurons developing in culture. We have found a strong correlation between the expression of the α subunit of the enzyme and the ability to undergo depolarization-dependent phosphorylation, which in young neurons is limited to the somatodendritic pool of the kinase. The lack of responsiveness of the axons of young αCaMKII-positive neurons is not caused by a lower Ca2+ influx but rather by a differential balance between kinase and phosphatase activities in this compartment. After the establishment of synaptic contacts, the presynaptic pool of the kinase displays an increasing level of activity and acquires the parallel ability to phosphorylate synapsin I, which represents one of the major CaMKII presynaptic targets in mature nerve terminals. In contrast, the activity of the postsynaptic pool of the kinase remains constant throughout synaptogenesis. In the presence of a nearly homogeneous subcellular distribution, this highly regionalized regulation of activity may reflect the multifunctional roles of CaMKII in both developing and mature neurons.

Original languageEnglish
Pages (from-to)7016-7026
Number of pages11
JournalJournal of Neuroscience
Volume22
Issue number16
Publication statusPublished - Aug 15 2002

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Phosphotransferases
Neurons
Synapsins
Phosphoric Monoester Hydrolases
Axons
Protein Isoforms
Phosphorylation
Enzymes

Keywords

  • Hippocampal neurons
  • Neuronal differentiation
  • Phosphospecific antibodies
  • Protein phosphorylation
  • Synapse
  • Synapsin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons. / Menegon, Andrea; Verderio, Claudia; Leoni, Chiara; Benfenati, Fabio; Czernik, Andrew J.; Greengard, Paul; Matteoli, Michela; Valtorta, Flavia.

In: Journal of Neuroscience, Vol. 22, No. 16, 15.08.2002, p. 7016-7026.

Research output: Contribution to journalArticle

Menegon A, Verderio C, Leoni C, Benfenati F, Czernik AJ, Greengard P et al. Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons. Journal of Neuroscience. 2002 Aug 15;22(16):7016-7026.
Menegon, Andrea ; Verderio, Claudia ; Leoni, Chiara ; Benfenati, Fabio ; Czernik, Andrew J. ; Greengard, Paul ; Matteoli, Michela ; Valtorta, Flavia. / Spatial and temporal regulation of Ca2+/calmodulin-dependent protein kinase II activity in developing neurons. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 16. pp. 7016-7026.
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