Spatial buffering during slow and paroxysmal sleep oscillations in cortical networks of glial cells in vivo

Florin Amzica, Marcello Massimini, Alfredo Manfridi

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of neuroglia to buffer local increases of extracellular K+ has been known from in vitro studies. This property may confer on these cells an active role in the modulation and spreading of cortical oscillatory activities. We addressed the question of the spatial buffering in vivo by performing single and double intraglial recordings, together with measures of the extracellular K+ and Ca2+ concentrations ([K+]out and [Ca2+]out) in the cerebral cortex of cats under ketamine and xylazine anesthesia during patterns of slow sleep oscillations and spike-wave seizures. In addition, we estimated the fluctuations of intraglial K+ concentrations ([K+]in). Measurements obtained during the slow oscillation indicated that glial cells phasically take up part of the extracellular K+ extruded by neurons during the depolarizing phase of the slow oscillation. During this condition, the redistribution of K+ appeared to be local. Large steady increases of [K+]out and phasic potassium accumulations were measured during spike-wave seizures. In this condition, [K+]in rose before [K+]out if the glial cells were located at some distance from the epileptic focus, suggesting faster K+ diffusion through the interglial syncytium. The simultaneously recorded [Ca2+]out dropped steadily during the seizures to levels incompatible with efficient synaptic transmission, but also displayed periodic oscillations, in phase with the intraseizure spike-wave complexes. In view of this fact, and considering the capability of K+ to modulate neuronal excitability both at the presynaptic and postsynaptic levels, we suggest that the K+ long-range spatial buffering operated by glia is a parallel synchronizing and/or spreading mechanism during paroxysmal oscillations.

Original languageEnglish
Pages (from-to)1042-1053
Number of pages12
JournalJournal of Neuroscience
Volume22
Issue number3
Publication statusPublished - Feb 1 2002

Keywords

  • Calcium
  • Epilepsy
  • Intracellular
  • Oscillations
  • Potassium
  • Sleep

ASJC Scopus subject areas

  • Neuroscience(all)

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