Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument

J. W. Sun, Y. Zhang, P. Rolfe, F. Scopesi, G. Serra, G. Wei

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We describe near infrared spectroscopy (NIRS) to study spatial variations in tissue oxygenation: oxyhaemoglobin (HbO2); deoxy-haemoglobin (HHb); and total haemoglobin (Hbtot). Apparatus was constructed incorporating light-emitting diodes (LED) as the light sources, sensitive photodiodes (PD) as the detectors, a virtual instrument to control the opto devices and collect and process the signals. The MATLAB script was embedded into LABVIEW to process and display the data. Changes in chromophore concentration were calculated using the modified Lambert-Beer Law together with the diffusion theory. We attached an LED array on the volar surface of the adult forearm with a PD detector placed at various distances of 12 to 40 mm from the sources. Further studies were performed in adults with forearm blood volume and oxygenation manipulated by venous and arterial occlusion respectively. Results indicated that source-detector spacing can achieve spatial sensitivity of blood volume and blood oxygenation measurement. The in vivo results demonstrated that the methodology and equipment achieved good performance as expected. In addition, we used dye-scatterer phantom to analyze the optical properties of near-infrared spectroscopy, where a spectrophotometer was used to calibrate the performance of the instrument. The in vitro results showed good correlation between the new instrument and the laboratory standard. The virtual instrument was a flexible and convenient means to develop apparatus for in vivo NIRS measurement. Spatial changes in tissue blood volume and oxygenation could be useful for clinical and research applications.

Original languageEnglish
Title of host publication2009 ICME International Conference on Complex Medical Engineering, CME 2009
DOIs
Publication statusPublished - 2009
Event2009 ICME International Conference on Complex Medical Engineering, CME 2009 - Tempe, AZ, United States
Duration: Apr 9 2009Apr 11 2009

Other

Other2009 ICME International Conference on Complex Medical Engineering, CME 2009
CountryUnited States
CityTempe, AZ
Period4/9/094/11/09

Fingerprint

Near infrared spectroscopy
Oxygenation
Near-Infrared Spectroscopy
Blood Volume
Spectrum Analysis
Blood
Tissue
Light
Forearm
Hemoglobin
Hemoglobins
Photodiodes
Detectors
Data Display
Light emitting diodes
Equipment and Supplies
Oxyhemoglobins
Spectrophotometers
Coloring Agents
Chromophores

Keywords

  • Diffuse reflectance
  • Lambert-Beer law
  • NIRS
  • Tissue oxygenation

ASJC Scopus subject areas

  • Biomedical Engineering
  • Health Informatics

Cite this

Sun, J. W., Zhang, Y., Rolfe, P., Scopesi, F., Serra, G., & Wei, G. (2009). Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument. In 2009 ICME International Conference on Complex Medical Engineering, CME 2009 [4906608] https://doi.org/10.1109/ICCME.2009.4906608

Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument. / Sun, J. W.; Zhang, Y.; Rolfe, P.; Scopesi, F.; Serra, G.; Wei, G.

2009 ICME International Conference on Complex Medical Engineering, CME 2009. 2009. 4906608.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sun, JW, Zhang, Y, Rolfe, P, Scopesi, F, Serra, G & Wei, G 2009, Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument. in 2009 ICME International Conference on Complex Medical Engineering, CME 2009., 4906608, 2009 ICME International Conference on Complex Medical Engineering, CME 2009, Tempe, AZ, United States, 4/9/09. https://doi.org/10.1109/ICCME.2009.4906608
Sun JW, Zhang Y, Rolfe P, Scopesi F, Serra G, Wei G. Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument. In 2009 ICME International Conference on Complex Medical Engineering, CME 2009. 2009. 4906608 https://doi.org/10.1109/ICCME.2009.4906608
Sun, J. W. ; Zhang, Y. ; Rolfe, P. ; Scopesi, F. ; Serra, G. ; Wei, G. / Spatial tissue oxygenation measurement with near infra-red spectroscopy using a virtual instrument. 2009 ICME International Conference on Complex Medical Engineering, CME 2009. 2009.
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