Spatiotemporal aeration and lung injury patterns are influenced by the first inflation strategy at birth

David G. Tingay, Anushi Rajapaksa, C. Elroy Zonneveld, Don Black, Elizabeth J. Perkins, Andy Adler, Bartlomiej Grychtol, Anna Lavizzari, Iné Z. Frerichs, Valerie A. Zahra, Peter G. Davis

Research output: Contribution to journalArticle

Abstract

Ineffective aeration during the first inflations at birth creates regional aeration and ventilation defects, initiating injurious pathways. This study aimed to compare a sustained first inflation at birth or dynamic end-expiratory supported recruitment during tidal inflations against ventilation without intentional recruitment on gas exchange, lung mechanics, spatiotemporal regional aeration and tidal ventilation, and regional lung injury in preterm lambs. Lambs (127 ± 2 d gestation), instrumented at birth, were ventilated for 60 minutes from birth with either lung-protective positive pressure ventilation (control) or as per control after either an initial 30 seconds of 40 cm H2O sustained inflation (SI) or an initial stepwise end-expiratory pressure recruitment maneuver during tidal inflations (duration 180 s; open lung ventilation [OLV]). At study completion, molecular markers of lung injury were analyzed. The initial use of an OLV maneuver, but not SI, at birth resulted in improved lung compliance, oxygenation, end-expiratory lung volume, and reduced ventilatory needs compared with control, persisting throughout the study. These changes were due to more uniform inter- and intrasubject gravitydependent spatiotemporal patterns of aeration (measured using electrical impedance tomography). Spatial distribution of tidal ventilation was more stable after either recruitment maneuver. All strategies caused regional lung injury patterns that mirrored associated regional volume states. Irrespective of strategy, spatiotemporal volume loss was consistently associated with upregulation of early growth response-1 expression. Our results show that mechanical and molecular consequences of lung aeration at birth are not simply related to rapidity of fluid clearance; they are also related to spatiotemporal pressure-volume interactions within the lung during inflation and deflation.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume54
Issue number2
DOIs
Publication statusPublished - Feb 1 2016

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Keywords

  • Aeration
  • Electrical impedance tomography
  • Infant, neonate
  • Lung injury
  • Lung mechanics

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Tingay, D. G., Rajapaksa, A., Zonneveld, C. E., Black, D., Perkins, E. J., Adler, A., Grychtol, B., Lavizzari, A., Frerichs, I. Z., Zahra, V. A., & Davis, P. G. (2016). Spatiotemporal aeration and lung injury patterns are influenced by the first inflation strategy at birth. American Journal of Respiratory Cell and Molecular Biology, 54(2), 263-272. https://doi.org/10.1165/rcmb.2015-0127OC