Species-specific monoclonal antibodies in the assignment of the gene for human fibronectin to chromosome 2.

L. Zardi, M. Cianfriglia, E. Balza, B. Carnemolla, A. Siri, C. M. Croce

Research output: Contribution to journalArticlepeer-review

Abstract

Using three different species-specific monoclonal antibodies we have studied, in human-mouse and human-hamster somatic cell hybrids, the correlation between the presence of different human chromosomes and the ability to release human fibronectin into the tissue culture medium. Presence of human fibronectin was determined by an affinity-radioimmunoassay. In addition, tissue culture media of the different hybrids were separated on SDS-polyacrylamide gels, the proteins were blotted onto a nitrocellulose sheet and human fibronectin visualized by an immunoenzymatic technique. Karyology and determination of isoenzyme markers of specific human chromosomes show that the ability to produce human fibronectin segregated with the presence of human chromosome 2.

Original languageEnglish
Pages (from-to)929-933
Number of pages5
JournalEMBO Journal
Volume1
Issue number8
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

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