Specific binding of 1-[3H]-O-alkyl-2-acetyl-sn-glyceryl-3-phosphoryl choline (platelet-activating factor, PAF) by human polymorphonuclear neutrophils

Federico Bussolino, Ciro Tetta, Giovanni Camussi

Research output: Contribution to journalArticle

Abstract

1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (PAF) is a potent activator of polymorphonuclear neutrophil (PMN) aggregation, exocytosis and chemotaxis. Specific desensitization of PMN to PAF suggests a receptor-mediated interaction. The binding of 1-[3H]-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (3H-PAF) to human PMN and platelets was analysed and compared. Binding was saturable at 0.6 n M and 0.1 n M for 2×106 PMN and 5×107 platelets, respectively. The time course of binding at 22°C and 37°C for both cell types reached the plateau at 2 min. The average Kd was 45.0±1.7 n M (mean ±1 SD of 4 experiments) for PMN (27.391±1381 sites for PMN) and 20.1±6.3 n M (4 experiments) for platelets (1577±461 sites for platelets). The Scatchard plot analysis revealed two distinct binding sites both on PMN and platelets: a high affinity binding site and a non-saturable binding site.

Original languageEnglish
Pages (from-to)15-17
Number of pages3
JournalAgents and Actions
Volume15
Issue number1-2
DOIs
Publication statusPublished - Aug 1984

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology (medical)

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