Specific Changes in the Posttranslational Regulation of Nucleolin in Lymphocytes from Patients Infected with Human Immunodeficiency Virus

Domenico Galati, Mirko Paiardini, Barbara Cervasi, Helmut Albrecht, Marialuisa Bocchino, Andrea Costantini, Maria Montroni, Mauro Magnani, Giuseppe Piedimonte, Guido Silvestri

Research output: Contribution to journalArticlepeer-review

Abstract

Lymphocytes isolated from human immunodeficiency virus (HIV)-infected patients have dysregulated cell-cycle control, consisting of increased activation of the cyclin B1/p34 cdc2 complex and abnormal nucleolar structure. To better characterize the molecular features of the HIV-associated cell-cycle perturbations, we performed a detailed analysis of the posttranslational regulation of nucleolin, a key structural protein in the nucleolus. We found that, in concanavalin A-stimulated lymphocytes from HIV-infected patients, the inappropriate activation of the cyclin B1/p34 cdc2 kinase complex is temporally associated with increased threonine phosphorylation, augmented fragmentation, and prominent extranuclear and cell-surface localization of nucleolin. Importantly, increased lymphocyte apoptosis is observed at the time of cell-surface localization of nucleolin. These results may delineate a direct molecular link between abnormal activation of cyclin B1/p34 cdc2 and the changes in the nucleolar structure, thus providing a better molecular definition of HIV-associated cell-cycle dysregulation.

Original languageEnglish
Pages (from-to)1483-1491
Number of pages9
JournalJournal of Infectious Diseases
Volume188
Issue number10
DOIs
Publication statusPublished - Nov 15 2003

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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