Specific interaction of plant HMG-like proteins with cruciform DNA

Marcella Bracale, Maria Grazia Galli, Christian Savini, Marco E. Bianchi

Research output: Contribution to journalArticlepeer-review

Abstract

Proteins which, on the basis of their solubility in 0.35% NaCl-2% TCA and of their electrophoretic mobility, correspond to animal HMG 1/2 family were isolated from nuclei of ungerminated pea embryos. These proteins ound with a high degree of specificity to synthetic cruciform DNA produced by annealing chemically synthesized oligonucleotides. Hence, specific binding to four-way junction DNA, previously reported for animal HMG 1 and 2 proteins proved also to be a property of plant HMG 1/2 family, in spite of their low homology to the animal ones.

Original languageEnglish
Pages (from-to)1493-1496
Number of pages4
JournalJournal of Experimental Botany
Volume45
Issue number10
DOIs
Publication statusPublished - Oct 1994

Keywords

  • Chromosomal proteins
  • Cruciform DNA
  • High mobility group proteins
  • Pisum sativum

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Plant Science

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