Specific mutations in the C-terminus domain of HBV surface antigen significantly correlate with low level of serum HBV-DNA in patients with chronic HBV infection

Carmen Mirabelli, Matteo Surdo, Formijn Van Hemert, Zhichao Lian, Romina Salpini, Valeria Cento, Maria Francesca Cortese, Marianna Aragri, Michela Pollicita, Claudia Alteri, Ada Bertoli, Ben Berkhout, Valeria Micheli, Guido Gubertini, Maria Mercedes Santoro, Sara Romano, Michela Visca, Martina Bernassola, Roberta Longo, Giuseppe Maria De SanctisPascal Trimoulet, Hervè Fleury, Nicoletta Marino, Francesco Mazzotta, Giuseppina Cappiello, Alberto Spanò, Cesare Sarrecchia, Jing Maria Zhang, Massimo Andreoni, Mario Angelico, Jens Verheyen, Carlo Federico Perno, Valentina Svicher

Research output: Contribution to journalArticle

Abstract

Background: To define HBsAg-mutations correlated with different serum HBV-DNA levels in HBV chronically-infected drug-naive patients. Methods: This study included 187 patients stratified into the following ranges of serum HBV-DNA:12-2000IU/ml, 2000-100,000IU/ml, and >100,000IU/ml. HBsAg-mutations were associated with HBV-DNA levels by applying a Bayesian-Partitional-Model and Fisher-exact test. Mutant and wild-type HBV genotype-D genomes were expressed in Huh7 cells and HBsAg-production was determined in cell-supernatants at 3 days-post-transfection. Results: Specific HBsAg-mutations (M197T,-S204N-Y206C/H-F220L) were significantly correlated with serum HBV-DNA 90%, P

Original languageEnglish
Pages (from-to)288-298
Number of pages11
JournalJournal of Infection
Volume70
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Keywords

  • HBsAg
  • HBV
  • Serum HBV-DNA
  • Virion maturation

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

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    Mirabelli, C., Surdo, M., Van Hemert, F., Lian, Z., Salpini, R., Cento, V., Cortese, M. F., Aragri, M., Pollicita, M., Alteri, C., Bertoli, A., Berkhout, B., Micheli, V., Gubertini, G., Santoro, M. M., Romano, S., Visca, M., Bernassola, M., Longo, R., ... Svicher, V. (2015). Specific mutations in the C-terminus domain of HBV surface antigen significantly correlate with low level of serum HBV-DNA in patients with chronic HBV infection. Journal of Infection, 70(3), 288-298. https://doi.org/10.1016/j.jinf.2014.10.015