Specific recognition of human CD3- CD16+ natural killer cells requires the expression of an autosomic recessive gene on target cells

Ermanno Ciccone, Daniela Pende, Oriane Viale, Giuseppe Tambussi, Silvano Ferrini, Roberto Biassoni, Anna Longo, John Guardiola, Alessandro Moretta, Lorenzo Moretta

Research output: Contribution to journalArticle

Abstract

We analyzed the recently defined ability of CD3-CD16+ cells to specifically recognize and lyse normal allogeneic target cells (PHA-induced blasts). The susceptibility to lysis by a given alloreactive natural killer (NK) clone ("1 anti-A") was expressed by PHA blasts derived from 9 of 38 random donors analyzed. In all instances, the specific lysis of "susceptible" target cells was >35% while that of "nonsusceptible" targets was -CD16+ clones could also be isolated from the reverse MLC combination. The relationship existing between lysis of normal allogeneic cells or tumor cells by the same CD3-CD16+ effector cell has been investigated: 1 anti-A specific CD3-CD16+ clones lysed PHA blasts of three of six cancer patients, while they lysed fresh tumor cells (ovarian carcinoma) from all six patients. The type of inheritance of the character "susceptibility to lysis" was analyzed in representative families. This analysis revealed that the character is inherited in an autosomic recessive fashion, and it is therefore different from MHC. We further investigated the type of segregation of the opposite character "resistance to lysis" (which is inherited in a dominant mode). The finding that this character segregated in all donors expressing given MHC haplotypes indicated that the gene regulating the expression of the NK-defined alloantigen is present on chromosome 6.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Experimental Medicine
Volume172
Issue number1
Publication statusPublished - Jul 1 1990

ASJC Scopus subject areas

  • Immunology

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