Specific role for protein kinase Cα in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts

Luisa Benussi, Stefano Govoni, Laura Gasparini, Giuliano Binetti, Marco Trabucchi, Angelo Bianchetti, Marco Racchi

Research output: Contribution to journalArticle

Abstract

Reduced levels of protein kinase Cα (PKCα) seems to be related to an altered amyloid precursor protein (APP) secretion in fibroblasts from Alzheimer's disease (AD) patients. In this report we used a specific inhibitor of PKCα (Go-6976), to investigate the role of PKCα in the basal and phorbol esters regulated secretion of soluble APP (sAPP) in human fibroblasts derived from healthy aged volunteers. Treatment with Go-6976 alone reduced basal secretion by a maximum of 39%, compared to untreated cells, suggesting the partial dependence of constitutive APP secretory pathway on PKCα enzyme. Moreover Go-6976 treatment completely abolished the effect of phorbol-esters mediated PKC stimulation on sAPP release, suggesting that PKCα is the only PKC isoform involved in controlling the secretion of sAPP in human fibroblasts.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalNeuroscience Letters
Volume240
Issue number2
DOIs
Publication statusPublished - Jan 9 1998

Keywords

  • Alzheimer's disease
  • Amyloid precursor protein
  • Human skin fibroblasts
  • Indolocarbazole Go-6976
  • Phorbol esters
  • Protein kinase C
  • Staurosporine

ASJC Scopus subject areas

  • Neuroscience(all)

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