Specificity of human T lymphocytes expressing a γ/δ T cell antigen receptor. Recognition of a polymorphic determinant of HLA class I molecules by a γ/δ clone

E. Ciccone, O. Viale, D. Pende, M. Malnati, G. Battista Ferrara, S. Barocci, A. Moretta, L. Moretta

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Alloreactive clones expressing T cell receptor (TcR) γ/δ were derived by limiting dilution from CD3+CD4-CD8-WT31- populations stimulated in allogeneic mixed lymphocyte culture. These clones specifically lysed phytohemagglutinin-induced blast cells bearing the stimulating alloantigens, whereas they had no effect on autologous or allogeneic unrelated target cells. Analysis of the reactivity with monoclonal antibodies (mAb) specific for two different subsets of TcR γ/δ (BB3 and δ-TCS-1) showed that five out of nine clones were BB3+, whereas the remaining reacted with δ-TCS-1. Therefore, we can conclude that both subsets of TcR γ/δ+ cells are able to specifically recognize and lyse allogeneic cells. mAb directed against the CD3-TcR γ/δ molecular complex strongly inhibited the specific cytolytic activity of TcR γ/δ+ clones, whereas they had no effect on the lysis of the natural killer-sensitive K-562 target cells mediated by the same clones. An alloreactive δ-TCS-1+ clone (LM12) was further characterized for its specificity. LM12 clone had been derived after stimulation in mixed lymphocyte culture against donor M.M. (HLA typing: Aw68, 24; B35, w55; DR1, 7). The analysis of a large panel of phytohemagglutinin-induced target cells revealed that only the HLA-A24+ target cells were lysed. The direct evidence that the A24 molecule represented the restriction element was provided by experiments using A24-transfected murine P815 target cells. Thus, clone LM12 efficiently lysed A24-transfected P815 cells, but not the same cells untransfected or transfected with the Cw3 gene. Therefore, it appears that polymorphic determinants of class I major histocompatibility complex molecules can be the target of TcR γ/δ+ alloreactive cell recognition.

Original languageEnglish
Pages (from-to)1267-1271
Number of pages5
JournalEuropean Journal of Immunology
Volume19
Issue number7
Publication statusPublished - 1989

Fingerprint

T-Cell Antigen Receptor
Clone Cells
T-Lymphocytes
Phytohemagglutinins
HLA-A24 Antigen
Monoclonal Antibodies
Lymphocytes
Histocompatibility Testing
Isoantigens
Major Histocompatibility Complex

ASJC Scopus subject areas

  • Immunology

Cite this

Specificity of human T lymphocytes expressing a γ/δ T cell antigen receptor. Recognition of a polymorphic determinant of HLA class I molecules by a γ/δ clone. / Ciccone, E.; Viale, O.; Pende, D.; Malnati, M.; Battista Ferrara, G.; Barocci, S.; Moretta, A.; Moretta, L.

In: European Journal of Immunology, Vol. 19, No. 7, 1989, p. 1267-1271.

Research output: Contribution to journalArticle

@article{f4cf22086c254f35bfe576dd4031088c,
title = "Specificity of human T lymphocytes expressing a γ/δ T cell antigen receptor. Recognition of a polymorphic determinant of HLA class I molecules by a γ/δ clone",
abstract = "Alloreactive clones expressing T cell receptor (TcR) γ/δ were derived by limiting dilution from CD3+CD4-CD8-WT31- populations stimulated in allogeneic mixed lymphocyte culture. These clones specifically lysed phytohemagglutinin-induced blast cells bearing the stimulating alloantigens, whereas they had no effect on autologous or allogeneic unrelated target cells. Analysis of the reactivity with monoclonal antibodies (mAb) specific for two different subsets of TcR γ/δ (BB3 and δ-TCS-1) showed that five out of nine clones were BB3+, whereas the remaining reacted with δ-TCS-1. Therefore, we can conclude that both subsets of TcR γ/δ+ cells are able to specifically recognize and lyse allogeneic cells. mAb directed against the CD3-TcR γ/δ molecular complex strongly inhibited the specific cytolytic activity of TcR γ/δ+ clones, whereas they had no effect on the lysis of the natural killer-sensitive K-562 target cells mediated by the same clones. An alloreactive δ-TCS-1+ clone (LM12) was further characterized for its specificity. LM12 clone had been derived after stimulation in mixed lymphocyte culture against donor M.M. (HLA typing: Aw68, 24; B35, w55; DR1, 7). The analysis of a large panel of phytohemagglutinin-induced target cells revealed that only the HLA-A24+ target cells were lysed. The direct evidence that the A24 molecule represented the restriction element was provided by experiments using A24-transfected murine P815 target cells. Thus, clone LM12 efficiently lysed A24-transfected P815 cells, but not the same cells untransfected or transfected with the Cw3 gene. Therefore, it appears that polymorphic determinants of class I major histocompatibility complex molecules can be the target of TcR γ/δ+ alloreactive cell recognition.",
author = "E. Ciccone and O. Viale and D. Pende and M. Malnati and {Battista Ferrara}, G. and S. Barocci and A. Moretta and L. Moretta",
year = "1989",
language = "English",
volume = "19",
pages = "1267--1271",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "wiley",
number = "7",

}

TY - JOUR

T1 - Specificity of human T lymphocytes expressing a γ/δ T cell antigen receptor. Recognition of a polymorphic determinant of HLA class I molecules by a γ/δ clone

AU - Ciccone, E.

AU - Viale, O.

AU - Pende, D.

AU - Malnati, M.

AU - Battista Ferrara, G.

AU - Barocci, S.

AU - Moretta, A.

AU - Moretta, L.

PY - 1989

Y1 - 1989

N2 - Alloreactive clones expressing T cell receptor (TcR) γ/δ were derived by limiting dilution from CD3+CD4-CD8-WT31- populations stimulated in allogeneic mixed lymphocyte culture. These clones specifically lysed phytohemagglutinin-induced blast cells bearing the stimulating alloantigens, whereas they had no effect on autologous or allogeneic unrelated target cells. Analysis of the reactivity with monoclonal antibodies (mAb) specific for two different subsets of TcR γ/δ (BB3 and δ-TCS-1) showed that five out of nine clones were BB3+, whereas the remaining reacted with δ-TCS-1. Therefore, we can conclude that both subsets of TcR γ/δ+ cells are able to specifically recognize and lyse allogeneic cells. mAb directed against the CD3-TcR γ/δ molecular complex strongly inhibited the specific cytolytic activity of TcR γ/δ+ clones, whereas they had no effect on the lysis of the natural killer-sensitive K-562 target cells mediated by the same clones. An alloreactive δ-TCS-1+ clone (LM12) was further characterized for its specificity. LM12 clone had been derived after stimulation in mixed lymphocyte culture against donor M.M. (HLA typing: Aw68, 24; B35, w55; DR1, 7). The analysis of a large panel of phytohemagglutinin-induced target cells revealed that only the HLA-A24+ target cells were lysed. The direct evidence that the A24 molecule represented the restriction element was provided by experiments using A24-transfected murine P815 target cells. Thus, clone LM12 efficiently lysed A24-transfected P815 cells, but not the same cells untransfected or transfected with the Cw3 gene. Therefore, it appears that polymorphic determinants of class I major histocompatibility complex molecules can be the target of TcR γ/δ+ alloreactive cell recognition.

AB - Alloreactive clones expressing T cell receptor (TcR) γ/δ were derived by limiting dilution from CD3+CD4-CD8-WT31- populations stimulated in allogeneic mixed lymphocyte culture. These clones specifically lysed phytohemagglutinin-induced blast cells bearing the stimulating alloantigens, whereas they had no effect on autologous or allogeneic unrelated target cells. Analysis of the reactivity with monoclonal antibodies (mAb) specific for two different subsets of TcR γ/δ (BB3 and δ-TCS-1) showed that five out of nine clones were BB3+, whereas the remaining reacted with δ-TCS-1. Therefore, we can conclude that both subsets of TcR γ/δ+ cells are able to specifically recognize and lyse allogeneic cells. mAb directed against the CD3-TcR γ/δ molecular complex strongly inhibited the specific cytolytic activity of TcR γ/δ+ clones, whereas they had no effect on the lysis of the natural killer-sensitive K-562 target cells mediated by the same clones. An alloreactive δ-TCS-1+ clone (LM12) was further characterized for its specificity. LM12 clone had been derived after stimulation in mixed lymphocyte culture against donor M.M. (HLA typing: Aw68, 24; B35, w55; DR1, 7). The analysis of a large panel of phytohemagglutinin-induced target cells revealed that only the HLA-A24+ target cells were lysed. The direct evidence that the A24 molecule represented the restriction element was provided by experiments using A24-transfected murine P815 target cells. Thus, clone LM12 efficiently lysed A24-transfected P815 cells, but not the same cells untransfected or transfected with the Cw3 gene. Therefore, it appears that polymorphic determinants of class I major histocompatibility complex molecules can be the target of TcR γ/δ+ alloreactive cell recognition.

UR - http://www.scopus.com/inward/record.url?scp=0024409716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024409716&partnerID=8YFLogxK

M3 - Article

C2 - 2527158

AN - SCOPUS:0024409716

VL - 19

SP - 1267

EP - 1271

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 7

ER -