Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein

Eduardo Nobile-Orazio, Arthur P. Hays, Norman Latov, Gayle Perman, Julia Golier, Michael E. Shy, Lorenza Freddo

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Some patients with neuropathy have IgM M-proteins that bind to myelin and to myelin-associated glycoprotein (MAG). We compared the binding properties of a human anti-MAG M-protein with three mouse monoclonal anti-MAG antibodies (GEN-Sl, GEN-S3, GEN-S8) and with a mouse monoclonal antibody (HNK-1) that binds to both MAG and to human natural killer cells. The antibodies GEN-Sl, GEN-S3, and GEN-S8 bound to different epitopes in the polypeptide portion of MAG as shown by peptide mapping, deglycosylation and competitive binding studies. The Mprotein.and HNK-1 bound to both CNS and PNS MAG and to several additional protein bands of 70K, 30K, 26K, and 23K daltons in peripheral, but not in central myelin; they did not bind to deglycosylated MAG. The M-protein and HNK-1 immunostained myelin diffusely, whereas GEN-S8 immunostained only the periaxonal and outer regions of myelin sheath, and there was no staining with GEN-Sl or GEN-S3. The human M-proteins probably bind to a carbohydrate moiety in MAG that is also present in other PNS myelin proteins. This may explain the observed differences in immunostaining and the sparing of the CNS in patients with anti-MAG M-proteins.

Original languageEnglish
Pages (from-to)1336-1342
Number of pages7
JournalNeurology
Volume34
Issue number10
Publication statusPublished - 1984

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Myelin-Associated Glycoprotein
Monoclonal Antibodies
Myelin Sheath
Proteins
Myelin Proteins
Peptide Mapping
Competitive Binding
Antibodies
Specificity
Protein
Mouse
Natural Killer Cells
Immunoglobulin M
Epitopes
Carbohydrates
Staining and Labeling
Peptides

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Nobile-Orazio, E., Hays, A. P., Latov, N., Perman, G., Golier, J., Shy, M. E., & Freddo, L. (1984). Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein. Neurology, 34(10), 1336-1342.

Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein. / Nobile-Orazio, Eduardo; Hays, Arthur P.; Latov, Norman; Perman, Gayle; Golier, Julia; Shy, Michael E.; Freddo, Lorenza.

In: Neurology, Vol. 34, No. 10, 1984, p. 1336-1342.

Research output: Contribution to journalArticle

Nobile-Orazio, E, Hays, AP, Latov, N, Perman, G, Golier, J, Shy, ME & Freddo, L 1984, 'Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein', Neurology, vol. 34, no. 10, pp. 1336-1342.
Nobile-Orazio E, Hays AP, Latov N, Perman G, Golier J, Shy ME et al. Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein. Neurology. 1984;34(10):1336-1342.
Nobile-Orazio, Eduardo ; Hays, Arthur P. ; Latov, Norman ; Perman, Gayle ; Golier, Julia ; Shy, Michael E. ; Freddo, Lorenza. / Specificity of mouse and human monoclonal antibodies to myelin-associated glycoprotein. In: Neurology. 1984 ; Vol. 34, No. 10. pp. 1336-1342.
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AU - Freddo, Lorenza

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