Abstract
A simple spectrophotometric method has been devised for determining the advanced products of glycation of lysine and albumin (browned compounds) based on their reactivity with nitro blue tetrazolium (NBT) salts. Aminoiminopropene derivatives of lysine and malondialdehyde, which share the same optical and fluorescence characteristics of browned compounds, were unreactive in the assay. Of the early products of glycation of lysine and albumin, the Schiff base adduct was unreactive in the assay, whereas the Amadori product (1-deoxy-1-morpholinofructose), when determined with NBT, gave a positive reaction that was slightly less marked than that of browned derivatives of albumin. It is concluded that the NBT assay is specific for Amadori and browned derivatives produced by glycation of amino groups and can be used for detecting such compounds in proteins (circulating and structural) exposed to high levels of carbohydrates.
| Original language | English |
|---|---|
| Pages (from-to) | 1101-1104 |
| Number of pages | 4 |
| Journal | Analyst |
| Volume | 113 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1988 |
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Keywords
- Browning
- Diabetes mellitus
- Glycation
- Nitro blue tetrazolium
- Spectrophotometry
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Electrochemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Spectrophotometric determination of browning products of glycation of protein amino groups based on their reactivity with nitro blue tetrazolium salts. / Ghiggeri, Gian Marco; Candiano, Giovanni; Ginevri, Fabrizio; Oleggini, Roberta; Piccardo, Maria Teresa; Bertelli, Roberta; Perfumo, Francesco; Gusmano, Rosanna.
In: Analyst, Vol. 113, No. 7, 1988, p. 1101-1104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spectrophotometric determination of browning products of glycation of protein amino groups based on their reactivity with nitro blue tetrazolium salts
AU - Ghiggeri, Gian Marco
AU - Candiano, Giovanni
AU - Ginevri, Fabrizio
AU - Oleggini, Roberta
AU - Piccardo, Maria Teresa
AU - Bertelli, Roberta
AU - Perfumo, Francesco
AU - Gusmano, Rosanna
PY - 1988
Y1 - 1988
N2 - A simple spectrophotometric method has been devised for determining the advanced products of glycation of lysine and albumin (browned compounds) based on their reactivity with nitro blue tetrazolium (NBT) salts. Aminoiminopropene derivatives of lysine and malondialdehyde, which share the same optical and fluorescence characteristics of browned compounds, were unreactive in the assay. Of the early products of glycation of lysine and albumin, the Schiff base adduct was unreactive in the assay, whereas the Amadori product (1-deoxy-1-morpholinofructose), when determined with NBT, gave a positive reaction that was slightly less marked than that of browned derivatives of albumin. It is concluded that the NBT assay is specific for Amadori and browned derivatives produced by glycation of amino groups and can be used for detecting such compounds in proteins (circulating and structural) exposed to high levels of carbohydrates.
AB - A simple spectrophotometric method has been devised for determining the advanced products of glycation of lysine and albumin (browned compounds) based on their reactivity with nitro blue tetrazolium (NBT) salts. Aminoiminopropene derivatives of lysine and malondialdehyde, which share the same optical and fluorescence characteristics of browned compounds, were unreactive in the assay. Of the early products of glycation of lysine and albumin, the Schiff base adduct was unreactive in the assay, whereas the Amadori product (1-deoxy-1-morpholinofructose), when determined with NBT, gave a positive reaction that was slightly less marked than that of browned derivatives of albumin. It is concluded that the NBT assay is specific for Amadori and browned derivatives produced by glycation of amino groups and can be used for detecting such compounds in proteins (circulating and structural) exposed to high levels of carbohydrates.
KW - Browning
KW - Diabetes mellitus
KW - Glycation
KW - Nitro blue tetrazolium
KW - Spectrophotometry
UR - http://www.scopus.com/inward/record.url?scp=0024046029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024046029&partnerID=8YFLogxK
U2 - 10.1039/AN9881301101
DO - 10.1039/AN9881301101
M3 - Article
C2 - 3146930
AN - SCOPUS:0024046029
VL - 113
SP - 1101
EP - 1104
JO - The Analyst
JF - The Analyst
SN - 0003-2654
IS - 7
ER -