Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins

A comparative study

Paolo Ascenzi, Massimo Coletta, Alessandro Desideri, Raffaele Petruzzelli, Francesca Polizio, Martino Bolognesi, Saverio Giovanni Condò, Bruno Giardina

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20°C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalJournal of Inorganic Biochemistry
Volume45
Issue number1
DOIs
Publication statusPublished - 1992

Fingerprint

Ruminants
Reindeer
Domestic Sheep
Horses
Nitric Oxide
Hemoglobins
Deer
Buffaloes
Derivatives
Paramagnetic resonance
Conformations
Chlorides
Buffers
Oxygen
glycosylated-nitric oxide complex hemoglobin A
musk

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins : A comparative study. / Ascenzi, Paolo; Coletta, Massimo; Desideri, Alessandro; Petruzzelli, Raffaele; Polizio, Francesca; Bolognesi, Martino; Condò, Saverio Giovanni; Giardina, Bruno.

In: Journal of Inorganic Biochemistry, Vol. 45, No. 1, 1992, p. 31-37.

Research output: Contribution to journalArticle

Ascenzi, P, Coletta, M, Desideri, A, Petruzzelli, R, Polizio, F, Bolognesi, M, Condò, SG & Giardina, B 1992, 'Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins: A comparative study', Journal of Inorganic Biochemistry, vol. 45, no. 1, pp. 31-37. https://doi.org/10.1016/0162-0134(92)84038-O
Ascenzi, Paolo ; Coletta, Massimo ; Desideri, Alessandro ; Petruzzelli, Raffaele ; Polizio, Francesca ; Bolognesi, Martino ; Condò, Saverio Giovanni ; Giardina, Bruno. / Spectroscopic properties of the nitric oxide derivative of ferrous man, horse, and ruminant hemoglobins : A comparative study. In: Journal of Inorganic Biochemistry. 1992 ; Vol. 45, No. 1. pp. 31-37.
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abstract = "The spectroscopic (EPR and absorbance) properties of the nitric oxide derivative of ferrous man, horse, buffalo, deer, mouflon, musk ox, ox, and reindeer hemoglobin (HbNO) have been investigated in the absence of any allosteric effector at pH 6.5 (in 0.1 M 2-[N-morpholino]ethanesulphonic acid/NaOH chloride-free buffer system), as well as at 100 K and/or 20°C. Man and horse HbNO show spectroscopic properties that are generally taken as typical of the high affinity state of ferrous tetrameric Hb's; on the other hand, the spectroscopic properties of ruminant (i.e., buffalo, deer, mouflon, musk ox, ox, and reindeer) HbNO are characteristic of the low affinity conformation. These results are in keeping with the functional properties of the mammalian Hb's considered and have been related to the peculiar low oxygen affinity of ruminant Hb's.",
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