Sperm cells as vectors for introducing foreign DNA into eggs: Genetic transformation of mice

Marialuisa Lavitrano, Antonella Camaioni, Vito M. Fazio, Susanna Dolci, Maria G. Farace, Corrado Spadafora

Research output: Contribution to journalArticle

Abstract

Mature mouse sperm cells incubated in an isotonic buffer with cloned DNA capture DNA molecules over a 15 min period. Spermatozoa incubated with pSV2CAT plasmid in either circular or linear form were used to fertilize mouse eggs in vitro. Sequences complementary to pSV2CAT were identified in ∼30% of 250 progeny by Southern blotting. A genomic library was constructed from the DNA of a positive mouse. Three positive clones were identified and two adjacent Hincll restriction fragments of 240 and 370 bp showed identical sequences to the corresponding fragments of the pSV2CAT plasmid. F1 progeny showed paternal and maternal transmission of the transgenes from founders. CAT gene expression was detected on tissues of adult F1 individuals, preferentially on tails and muscle. We conclude that transgenic mice can be obtained using sperm cells as foreign DNA vectors.

Original languageEnglish
Pages (from-to)717-723
Number of pages7
JournalCell
Volume57
Issue number5
DOIs
Publication statusPublished - Jun 2 1989

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Sperm cells as vectors for introducing foreign DNA into eggs: Genetic transformation of mice'. Together they form a unique fingerprint.

  • Cite this