Sperm protein 17 is expressed in the sperm fibrous sheath

Maurizio Chiriva-Internati, Nicoletta Gagliano, Elena Donetti, Francesco Costa, Fabio Grizzi, Barbara Franceschini, Elena Albani, Paolo E. Levi-Setti, Magda Gioia, Marjorie Jenkins, Everardo Cobos, W. Martin Martin

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Sperm protein 17 (Sp17) is a highly conserved mammalian protein characterized in rabbit, mouse, monkey, baboon, macaque, human testis and spermatozoa. mRNA encoding Sp17 has been detected in a range of murine and human somatic tissues. It was also recognized in two myeloma cell lines and in neoplastic cells from patients with multiple myeloma and ovarian carcinoma. These data all indicate that Sp17 is widely distributed in humans, expressed not only in germinal cells and in a variety of somatic tissues, but also in neoplastic cells of unrelated origin. Methods: Sp17 expression was analyzed by immunocytochemistry and transmission electron microscopy on spermatozoa. Results: Here, we demonstrate the ultrastructural localization of human Sp17 throughout the spermatozoa flagellar fibrous sheath, and its presence in spermatozoa during in vitro states from their ejaculation to the oocyte fertilization. Conclusion: These findings suggest a possible role of Sp17 in regulating sperm maturation, capacitation, acrosomal reaction and interactions with the oocyte zona pellucida during the fertilization process. Further, the high degree of sequence conservation throughout its N-terminal half, and the presence of an A-kinase anchoring protein (AKAP)-binding motif within this region, suggest that Sp17 might play a regulatory role in a protein kinase A-independent AKAP complex in both germinal and somatic cells.

Original languageEnglish
Article number61
JournalJournal of Translational Medicine
Volume7
DOIs
Publication statusPublished - Jul 15 2009

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Spermatozoa
Proteins
Fertilization
Phosphotransferases
Oocytes
Tissue
Sperm Capacitation
Sperm Maturation
Zona Pellucida
Cyclic AMP-Dependent Protein Kinases
Amino Acid Motifs
Ejaculation
Papio
Macaca
Conservation
Multiple Myeloma
Transmission Electron Microscopy
Protein Binding
Protein Kinases
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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Sperm protein 17 is expressed in the sperm fibrous sheath. / Chiriva-Internati, Maurizio; Gagliano, Nicoletta; Donetti, Elena; Costa, Francesco; Grizzi, Fabio; Franceschini, Barbara; Albani, Elena; Levi-Setti, Paolo E.; Gioia, Magda; Jenkins, Marjorie; Cobos, Everardo; Martin, W. Martin.

In: Journal of Translational Medicine, Vol. 7, 61, 15.07.2009.

Research output: Contribution to journalArticle

Chiriva-Internati, M, Gagliano, N, Donetti, E, Costa, F, Grizzi, F, Franceschini, B, Albani, E, Levi-Setti, PE, Gioia, M, Jenkins, M, Cobos, E & Martin, WM 2009, 'Sperm protein 17 is expressed in the sperm fibrous sheath', Journal of Translational Medicine, vol. 7, 61. https://doi.org/10.1186/1479-5876-7-61
Chiriva-Internati, Maurizio ; Gagliano, Nicoletta ; Donetti, Elena ; Costa, Francesco ; Grizzi, Fabio ; Franceschini, Barbara ; Albani, Elena ; Levi-Setti, Paolo E. ; Gioia, Magda ; Jenkins, Marjorie ; Cobos, Everardo ; Martin, W. Martin. / Sperm protein 17 is expressed in the sperm fibrous sheath. In: Journal of Translational Medicine. 2009 ; Vol. 7.
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AU - Albani, Elena

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