Spheres derived from lung adenocarcinoma pleural effusions: Molecular characterization and tumor engraftment

Rita Mancini, Enrico Giarnieri, Claudia de Vitis, Donatella Malanga, Giuseppe Roscilli, Alessia Noto, Emanuele Marra, Carmelo Laudanna, Pietro Zoppoli, Pasquale de Luca, Andrea Affuso, Luigi Ruco, Arianna Di Napoli, Giuseppe Mesiti, Luigi Aurisicchio, Alberto Ricci, Salvatore Mariotta, Lara Pisani, Claudio Andreetti, Giuseppe VigliettoErino A. Rendina, Maria Rosaria Giovagnoli, Gennaro Ciliberto

Research output: Contribution to journalArticlepeer-review


Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in vitro cultures both in adherent and/or in spheroid conditions in almost all cases analyzed. We characterized in greater detail two samples which showed the most efficient propagation in vitro. In these samples we also compared gene profiles of spheroid vs adherent cultures and identified a set of differentially expressed genes. Finally we achieved efficient tumor engraftment in recipient NOD/SCID mice, also upon inoculation of small number of cells, thus suggesting indirectly the presence of tumor initiating cells.

Original languageEnglish
Article numbere21320
JournalPLoS One
Issue number7
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)


Dive into the research topics of 'Spheres derived from lung adenocarcinoma pleural effusions: Molecular characterization and tumor engraftment'. Together they form a unique fingerprint.

Cite this