Sphingoid bioregulators in the differentiation of cells of neural origin

Guido Tettamanti, Alessandro Prinetti, Rosaria Bassi, Paola Viani, Paola Giussani, Laura Riboni

Research output: Contribution to journalArticle


The involvement of ceramide in the differentiation of two neuroblastoma cell lines, Neuro2a and SH-SY5Y, and cerebellar granule cells in primary culture was investigated. The following results were obtained: (a) the cellular content of ceramide markedly increased with induced differentiation of Neuro2a cells (inducers: RA, FCS deprivation), SH-SY5Y cells (inducers: RA, PMA), and spontaneous differentiation of cerebellar granule cells; (b) all the investigated cells in the differentiated form displayed a higher ability to produce ceramide from exogenously administered [3H]Sph-SM and expressed a higher content of neutral sphingomyelinase and, in the case of cerebellar granule cells, also of acidic sphingomyelinase; (c) inhibition of ceramide biosynthesis by Fumonisin B1 blocked the process of differentiation in Neuro2a and cerebellar granule cells; and (d) treatments capable of enhancing ceramide level (administration of sphingosine or C2-Ceramide) induced differentiation in both Neuro2a and SH-SY5Y cells. The data obtained support the notion that ceramide plays a general biomodulatory role in neural cell differentiation.

Original languageEnglish
Pages (from-to)263-275
Number of pages13
JournalJournal of Lipid Mediators and Cell Signalling
Issue number1-3
Publication statusPublished - Sep 1996


  • ceramide
  • cultured neurons
  • neural differentiation
  • neuroblastoma cells
  • sphingoid bioregulators

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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    Tettamanti, G., Prinetti, A., Bassi, R., Viani, P., Giussani, P., & Riboni, L. (1996). Sphingoid bioregulators in the differentiation of cells of neural origin. Journal of Lipid Mediators and Cell Signalling, 14(1-3), 263-275. https://doi.org/10.1016/0929-7855(96)00535-4