Sphingosine-1-phosphate stimulates cortisol secretion

Miriam Rábano, Ana Peña, Leyre Brizuela, Aida Marino, José María Macarulla, Miguel Trueba, Antonio Gómez-Muñoz, Guido Tettamanti

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We show here for the first time that sphingosine-1-phosphate (Sph-1-P) stimulates cortisol secretion in zona fasciculata cells of bovine adrenal glands. This effect was dependent upon protein kinase C (PKC) and extracellular Ca2+, and was inhibited by pertussis toxin. Sph-1-P activated phospholipase D (PLD) through a pertussis toxin-sensitive mechanism, also involving extracellular Ca2+ and PKC. Primary alcohols, which attenuate formation of phosphatidic acid (the product of PLD), and cell-permeable ceramides, which inhibit PLD, blocked Sph-1-P-induced cortisol secretion. In conclusion, Sph-1-P stimulates cortisol secretion through a mechanism involving Gi/o protein-coupled receptors, extracellular Ca2+, PKC and PLD.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEBS Letters
Volume535
Issue number1-3
DOIs
Publication statusPublished - Jan 30 2003

Fingerprint

Phospholipase D
Hydrocortisone
Protein Kinase C
Pertussis Toxin
Zona Fasciculata
Phosphatidic Acids
Somatostatin-Secreting Cells
Ceramides
Adrenal Glands
Alcohols
sphingosine 1-phosphate
Proteins

Keywords

  • Adrenal gland
  • Ceramide
  • Cortisol secretion
  • Phospholipase D
  • Protein kinase C
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Rábano, M., Peña, A., Brizuela, L., Marino, A., Macarulla, J. M., Trueba, M., ... Tettamanti, G. (2003). Sphingosine-1-phosphate stimulates cortisol secretion. FEBS Letters, 535(1-3), 101-105. https://doi.org/10.1016/S0014-5793(02)03882-6

Sphingosine-1-phosphate stimulates cortisol secretion. / Rábano, Miriam; Peña, Ana; Brizuela, Leyre; Marino, Aida; Macarulla, José María; Trueba, Miguel; Gómez-Muñoz, Antonio; Tettamanti, Guido.

In: FEBS Letters, Vol. 535, No. 1-3, 30.01.2003, p. 101-105.

Research output: Contribution to journalArticle

Rábano, M, Peña, A, Brizuela, L, Marino, A, Macarulla, JM, Trueba, M, Gómez-Muñoz, A & Tettamanti, G 2003, 'Sphingosine-1-phosphate stimulates cortisol secretion', FEBS Letters, vol. 535, no. 1-3, pp. 101-105. https://doi.org/10.1016/S0014-5793(02)03882-6
Rábano M, Peña A, Brizuela L, Marino A, Macarulla JM, Trueba M et al. Sphingosine-1-phosphate stimulates cortisol secretion. FEBS Letters. 2003 Jan 30;535(1-3):101-105. https://doi.org/10.1016/S0014-5793(02)03882-6
Rábano, Miriam ; Peña, Ana ; Brizuela, Leyre ; Marino, Aida ; Macarulla, José María ; Trueba, Miguel ; Gómez-Muñoz, Antonio ; Tettamanti, Guido. / Sphingosine-1-phosphate stimulates cortisol secretion. In: FEBS Letters. 2003 ; Vol. 535, No. 1-3. pp. 101-105.
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