Sphingosine-1-phosphate stimulates cortisol secretion

Miriam Rábano, Ana Peña, Leyre Brizuela, Aida Marino, José María Macarulla, Miguel Trueba, Antonio Gómez-Muñoz, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

We show here for the first time that sphingosine-1-phosphate (Sph-1-P) stimulates cortisol secretion in zona fasciculata cells of bovine adrenal glands. This effect was dependent upon protein kinase C (PKC) and extracellular Ca2+, and was inhibited by pertussis toxin. Sph-1-P activated phospholipase D (PLD) through a pertussis toxin-sensitive mechanism, also involving extracellular Ca2+ and PKC. Primary alcohols, which attenuate formation of phosphatidic acid (the product of PLD), and cell-permeable ceramides, which inhibit PLD, blocked Sph-1-P-induced cortisol secretion. In conclusion, Sph-1-P stimulates cortisol secretion through a mechanism involving Gi/o protein-coupled receptors, extracellular Ca2+, PKC and PLD.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEBS Letters
Volume535
Issue number1-3
DOIs
Publication statusPublished - Jan 30 2003

Keywords

  • Adrenal gland
  • Ceramide
  • Cortisol secretion
  • Phospholipase D
  • Protein kinase C
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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